FASEB J 2017 Mar 01;313:1141-1152. doi: 10.1096/fj.201600909R.

LRGUK1 is part of a multiprotein complex required for manchette function and male fertility.

???displayArticle.abstract???
Infertility occurs in 1 in 20 young men and is idiopathic in origin in most. We have reported that the leucine-rich repeat (LRR) and guanylate kinase-like domain containing, isoform (LRGUK)-1 is essential for sperm head shaping, via the manchette, and the initiation of sperm tail growth from the centriole/basal body, and thus, male fertility. Within this study we have used a yeast 2-hybrid screen of an adult testis library to identify LRGUK1-binding partners, which were then validated with a range of techniques. The data indicate that LRGUK1 likely achieves its function in partnership with members of the HOOK family of proteins (HOOK-1-3), Rab3-interacting molecule binding protein (RIMBP)-3 and kinesin light chain (KLC)-3, all of which are associated with intracellular protein transport as cargo adaptor proteins and are localized to the manchette. LRGUK1 consists of 3 domains; an LRR, a guanylate kinase (GUK)-like and an unnamed domain. In the present study, we showed that the GUK-like domain is essential for binding to HOOK2 and RIMBP3, and the LRR domain is essential for binding to KLC3. These findings establish LRGUK1 as a key component of a multiprotein complex with an essential role in microtubule dynamics within haploid male germ cells.-Okuda, H., DeBoer, K., O''Connor, A. E., Merriner, D. J., Jamsai, D., O''Bryan, M. K. LRGUK1 is part of a multiprotein complex required for manchette function and male fertility.

???displayArticle.pubmedLink??? 28003339
???displayArticle.link??? FASEB J


Genes referenced: guk1 LOC100890978 LOC590870 LOC591902 LOC593824 LOC594566 lrguk rab3