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Sci Rep
2016 Feb 22;6:21554. doi: 10.1038/srep21554.
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Ocean acidification increases copper toxicity differentially in two key marine invertebrates with distinct acid-base responses.
Lewis C
,
Ellis RP
,
Vernon E
,
Elliot K
,
Newbatt S
,
Wilson RW
.
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Ocean acidification (OA) is expected to indirectly impact biota living in contaminated coastal environments by altering the bioavailability and potentially toxicity of many pH-sensitive metals. Here, we show that OA (pH 7.71; pCO2 1480 μatm) significantly increases the toxicity responses to a global coastal contaminant (copper ~0.1 μM) in two keystone benthic species; mussels (Mytilus edulis) and purple sea urchins (Paracentrotus lividus). Mussels showed an extracellular acidosis in response to OA and copper individually which was enhanced during combined exposure. In contrast, urchins maintained extracellular fluid pH under OA by accumulating bicarbonate but exhibited a slight alkalosis in response to copper either alone or with OA. Importantly, copper-induced damage to DNA and lipids was significantly greater under OA compared to control conditions (pH 8.14; pCO2 470 μatm) for both species. However, this increase in DNA-damage was four times lower in urchins than mussels, suggesting that internal acid-base regulation in urchins may substantially moderate the magnitude of this OA-induced copper toxicity effect. Thus, changes in metal toxicity under OA may not purely be driven by metal speciation in seawater and may be far more diverse than either single-stressor or single-species studies indicate. This has important implications for future environmental management strategies.
Figure 1. Acid-base parameters in the haemolymph of Mytilus edulis (a,c,e) and coelomic fluid of Paracentrotus lividus (b,d,f) following 14 day exposures to elevated pCO2 with and without the presence of nominal 0.1 μM copper; (a,b) haemolymph/coelomic fluid pH, (c,d) haemolymph/coelomic fluid bicarbonate concentrations, and (e,f) haemolymph/coelomic fluid pCO2. [N.B. *represent significant differences].
Figure 2. Davenport diagram illustrating the relationship between pH, bicarbonate and pCO2 in the haemolymph and coelomic fluid of (a) Mytilus edulis and (b) Paracentrotus lividus respectively. Lines represent isopleths of equal pCO2 (mmHg). Position calculated from means ± SEM for haemolymph/coelomic fluid pH and [HCO3−] according to pK1 values calculated from57.
Figure 3. Oxidative stress indicators in the mussel Mytilus edulis (a,c,e) and the adult purple urchin Paracentrotus lividus (b,d,f) following 14 day exposures to elevated pCO2 with and without the presence of nominal 0.1 μM copper; (a,b) Activity of the anti-oxidant enzyme superoxide dismutase (SOD) activity; (c,d) lipid peroxidation measured as malondialdehyde (MDA) levels; (e,f) DNA damage, measured as percentage of single strand breaks in haemocytes/coelomocytes.
Bielmyer,
The effects of metals on embryo-larval and adult life stages of the sea urchin, Diadema antillarum.
2005, Pubmed,
Echinobase
Bielmyer,
The effects of metals on embryo-larval and adult life stages of the sea urchin, Diadema antillarum.
2005,
Pubmed
,
Echinobase
Bryan,
Bioavailability, accumulation and effects of heavy metals in sediments with special reference to United Kingdom estuaries: a review.
1992,
Pubmed
Byrne,
Multistressor impacts of warming and acidification of the ocean on marine invertebrates' life histories.
2013,
Pubmed
Camejo,
Analysis of oxidation and antioxidants using microtiter plates.
1998,
Pubmed
Campbell,
Ocean acidification increases copper toxicity to the early life history stages of the polychaete Arenicola marina in artificial seawater.
2014,
Pubmed
Catarino,
Acid-base balance and metabolic response of the sea urchin Paracentrotus lividus to different seawater pH and temperatures.
2012,
Pubmed
,
Echinobase
Collard,
Buffer capacity of the coelomic fluid in echinoderms.
2013,
Pubmed
,
Echinobase
Crain,
Interactive and cumulative effects of multiple human stressors in marine systems.
2008,
Pubmed
Fabricius,
Ecological effects of ocean acidification and habitat complexity on reef-associated macroinvertebrate communities.
2014,
Pubmed
,
Echinobase
Fdil,
Valve movement response of the mussel mytilus galloprovincialis to metals (Cu, Hg, Cd and Zn) and phosphate industry effluents from Moroccan Atlantic coast.
2006,
Pubmed
Gattuso,
OCEANOGRAPHY. Contrasting futures for ocean and society from different anthropogenic CO₂ emissions scenarios.
2015,
Pubmed
Götze,
Interactive effects of CO₂ and trace metals on the proteasome activity and cellular stress response of marine bivalves Crassostrea virginica and Mercenaria mercenaria.
2014,
Pubmed
Halpern,
A global map of human impact on marine ecosystems.
2008,
Pubmed
Ivanina,
Short-term acute hypercapnia affects cellular responses to trace metals in the hard clams Mercenaria mercenaria.
2013,
Pubmed
Jing,
Metal accumulation and enzyme activities in gills and digestive gland of pearl oyster (Pinctada fucata) exposed to copper.
2006,
Pubmed
Jones,
Copper speciation survey from UK marinas, harbours and estuaries.
2007,
Pubmed
Kroeker,
Impacts of ocean acidification on marine organisms: quantifying sensitivities and interaction with warming.
2013,
Pubmed
Lacoue-Labarthe,
Effects of ocean acidification on trace element accumulation in the early-life stages of squid Loligo vulgaris.
2011,
Pubmed
Lewis,
Genotoxic damage in polychaetes: a study of species and cell-type sensitivities.
2008,
Pubmed
Maria,
Antioxidant and lipid peroxidation responses in Mytilus galloprovincialis exposed to mixtures of benzo(a)pyrene and copper.
2011,
Pubmed
Orr,
Anthropogenic ocean acidification over the twenty-first century and its impact on calcifying organisms.
2005,
Pubmed
Parry,
Interactive effects of temperature and copper on immunocompetence and disease susceptibility in mussels (Mytilus edulis).
2004,
Pubmed
Pascal,
The toxicological interaction between ocean acidity and metals in coastal meiobenthic copepods.
2010,
Pubmed
Przeslawski,
A review and meta-analysis of the effects of multiple abiotic stressors on marine embryos and larvae.
2015,
Pubmed
Rhee,
Expression of superoxide dismutase (SOD) genes from the copper-exposed polychaete, Neanthes succinea.
2011,
Pubmed
Roberts,
Ocean acidification increases the toxicity of contaminated sediments.
2013,
Pubmed
Schiff,
Extent and magnitude of copper contamination in marinas of the San Diego region, California, USA.
2007,
Pubmed
Stohs,
Oxidative mechanisms in the toxicity of metal ions.
1995,
Pubmed
Truchot,
Carbon dioxide combining properties of the blood of the shore crab Carcinus maenas (L): carbon dioxide solubility coefficient and carbonic acid dissociation constants.
1976,
Pubmed
Wang,
Copper exposure impairs intra- and extracellular acid-base regulation during hypercapnia in the fresh water rainbow trout (Oncorhynchus mykiss).
1998,
Pubmed
