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J Fluoresc
2013 Nov 01;236:1147-55. doi: 10.1007/s10895-013-1244-4.
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Kinetics and thermodynamics of glycans and glycoproteins binding to Holothuria scabra lectin: a fluorescence and surface plasmon resonance spectroscopic study.
Gowda NM
,
Gaikwad SM
,
Khan MI
.
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Holothuria scabra produces a monomeric lectin (HSL) of 182 kDa. HSL showed strong antibacterial activity and induced bacterial agglutination under in vitro conditions, indicating its role in animals'' innate immune responses. Very few lectins have been reported from echinoderms and none of these lectins have been explored in detail for their sugar-binding kinetics. Affinity, kinetics and thermodynamic analysis of glycans and glycoproteins binding to HSL were studied by fluorescence and surface plasmon resonance spectroscopy. Lectin binds with higher affinity to O-linked than N-linked asialo glycans, and the affinities were relatively higher than that for sialated glycans and glycoproteins. T-antigen α-methyl glycoside was the most potent ligand having the highest affinity (Ka 8.32 ×10(7) M(-1)). Thermodynamic and kinetic analysis indicated that the binding of galactosyl Tn-antigen and asialo glycans is accompanied by an enthalpic contribution in addition to higher association rate coupled by low activation energy for the association process. Presence of sialic acid or protein matrix inhibits binding. Higher affinity of HSL for O-glycans than N-glycans had biological implications; since HSL specifically recognizes bacteria, which have mucin or O-glycan cognate on their cell surfaces and play a major role in animal innate immunity. Since, HSL had higher affinity to T-antigen, makes it a useful tool for cancer diagnostic purpose.
Bachhawat,
On the stringent requirement of mannosyl substitution in mannooligosaccharides for the recognition by garlic (Allium sativum) lectin. A Surface Plasmon Resonance Study.
2001, Pubmed
Bachhawat,
On the stringent requirement of mannosyl substitution in mannooligosaccharides for the recognition by garlic (Allium sativum) lectin. A Surface Plasmon Resonance Study.
2001,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bulgakov,
Isolation and properties of a mannan-binding lectin from the coelomic fluid of the holothurian Cucumaria japonica.
2000,
Pubmed
,
Echinobase
Chipman,
The binding of oligosaccharides containing N-acetylglucosamine and N-acetylmuramic acid to lysozyme. The specificity of binding subsites.
1967,
Pubmed
Clegg,
Binding of 4-methylumbelliferyl alpha-D-mannopyranoside to dimeric concanavalin A: fluorescence temperature-jump relaxation study.
1977,
Pubmed
Cooper,
Invertebrate immunity: another viewpoint.
1992,
Pubmed
Danguy,
Applications of lectins and neoglycoconjugates in histology and pathology.
1998,
Pubmed
Gowda,
Purification and characterization of a T-antigen specific lectin from the coelomic fluid of a marine invertebrate, sea cucumber (Holothuria scabra).
2008,
Pubmed
,
Echinobase
Gowda,
T-antigen binding lectin with antibacterial activity from marine invertebrate, sea cucumber (Holothuria scabra): possible involvement in differential recognition of bacteria.
2008,
Pubmed
,
Echinobase
Hassanein,
Thomsen-Friedenreich (T) antigen: a possible tool for differentiating sebaceous carcinoma from its simulators.
2001,
Pubmed
Hatakeyama,
Purification and characterization of two lectins from the sea cucumber Stichopus japonicus.
1993,
Pubmed
,
Echinobase
Hatakeyama,
Galactose recognition by a tetrameric C-type lectin, CEL-IV, containing the EPN carbohydrate recognition motif.
2011,
Pubmed
,
Echinobase
Hatakeyama,
Purification and characterization of four Ca(2+)-dependent lectins from the marine invertebrate, Cucumaria echinata.
1994,
Pubmed
,
Echinobase
Kamiya,
Lectins in the hemolymph of a starfish, Asterina pectinifera: purification and characterization.
1992,
Pubmed
,
Echinobase
Kanitakis,
Differential expression of the cancer associated antigens T (Thomsen-Friedenreich) and Tn to the skin in primary and metastatic carcinomas.
1998,
Pubmed
Kelly,
The use of circular dichroism in the investigation of protein structure and function.
2000,
Pubmed
Khan,
Purification and characterization of a lectin from endophytic fungus Fusarium solani having complex sugar specificity.
2007,
Pubmed
Lee,
Fluorescence spectrometry in studies of carbohydrate-protein interactions.
1997,
Pubmed
Lis,
Soybean hemagglutinin, a plant glycoprotein. I. Isolation of a glycopeptide.
1966,
Pubmed
Lis,
Lectins as molecules and as tools.
1986,
Pubmed
Maehashi,
Identification of the sea urchin 350-kDa sperm-binding protein as a new sialic acid-binding lectin that belongs to the heat shock protein 110 family: implication of its binding to gangliosides in sperm lipid rafts in fertilization.
2003,
Pubmed
,
Echinobase
Matsui,
Purification and characterization of two Ca(2+)-dependent lectins from coelomic plasma of sea cucumber, Stichopus japonicus.
1994,
Pubmed
,
Echinobase
Pavliak,
Binding of the O-antigen of Shigella dysenteriae type 1 and 26 related synthetic fragments to a monoclonal IgM antibody.
1993,
Pubmed
Schuster,
Assembly and function of a quaternary signal transduction complex monitored by surface plasmon resonance.
1993,
Pubmed
Sharon,
Lectins as cell recognition molecules.
1989,
Pubmed
Smith,
Echinoderm immunity.
2010,
Pubmed
,
Echinobase
Stabili,
Antibacterial activity in the coelomocytes of the sea urchin Paracentrotus lividus.
1996,
Pubmed
,
Echinobase
Tateno,
SUEL-related lectins, a lectin family widely distributed throughout organisms.
2010,
Pubmed
,
Echinobase
Thakur,
Complex carbohydrate specificity of lectin from fruiting body of Ganoderma lucidum. A surface plasmon resonance study.
2007,
Pubmed
Townsend,
Binding of N-linked bovine fetuin glycopeptides to isolated rabbit hepatocytes: Gal/GalNAc hepatic lectin discrimination between Gal beta(1,4)GlcNAc and Gal beta(1,3)GlcNAc in a triantennary structure.
1986,
Pubmed
Vinogradov,
The structures of the carbohydrate backbones of the lipopolysaccharides from Escherichia coli rough mutants F470 (R1 core type) and F576 (R2 core type).
1999,
Pubmed
Vitale,
Near-ultraviolet magnetic circular dichroism spectroscopy of protein conformational states: correlation of tryptophan band position and intensity with hemoglobin allostery.
2000,
Pubmed
van de Weert,
Fluorescence quenching to study protein-ligand binding: common errors.
2010,
Pubmed
