Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Development
2012 Feb 01;1394:783-92. doi: 10.1242/dev.076752.
Show Gene links
Show Anatomy links
Programmed reduction of ABC transporter activity in sea urchin germline progenitors.
Campanale JP
,
Hamdoun A
.
???displayArticle.abstract??? ATP-binding cassette (ABC) transporters protect embryos and stem cells from mutagens and pump morphogens that control cell fate and migration. In this study, we measured dynamics of ABC transporter activity during formation of sea urchin embryonic cells necessary for the production of gametes, termed the small micromeres. Unexpectedly, we found small micromeres accumulate 2.32 times more of the ABC transporter substrates calcein-AM, CellTrace RedOrange, BoDipy-verapamil and BoDipy-vinblastine, than any other cell in the embryo, indicating a reduction in multidrug efflux activity. The reduction in small micromereABC transporter activity is mediated by a pulse of endocytosis occurring 20-60 minutes after the appearance of the micromeres--the precursors of the small micromeres. Treating embryos with phenylarsine oxide, an inhibitor of endocytosis, prevents the reduction of transporter activity. Tetramethylrhodamine dextran and cholera toxin B uptake experiments indicate that micromeres have higher rates of bulk and raft-associated membrane endocytosis during the window of transporter downregulation. We hypothesized that this loss of efflux transport could be required for the detection of developmental signaling molecules such as germ cell chemoattractants. Consistent with this hypothesis, we found that the inhibition of ABCB and ABCC-types of efflux transporters disrupts the ordered distribution of small micromeres to the left and right coelomic pouches. These results point to tradeoffs between signaling and the protective functions of the transporters.
Alford,
Cell polarity emerges at first cleavage in sea urchin embryos.
2009, Pubmed,
Echinobase
Alford,
Cell polarity emerges at first cleavage in sea urchin embryos.
2009,
Pubmed
,
Echinobase
Aller,
Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding.
2009,
Pubmed
Anjard,
Signal transduction pathways leading to spore differentiation in Dictyostelium discoideum.
1998,
Pubmed
Bunting,
ABC transporters as phenotypic markers and functional regulators of stem cells.
2002,
Pubmed
Cameron,
Macromere cell fates during sea urchin development.
1991,
Pubmed
,
Echinobase
Christie,
phot1 inhibition of ABCB19 primes lateral auxin fluxes in the shoot apex required for phototropism.
2011,
Pubmed
Conner,
rab3 mediates cortical granule exocytosis in the sea urchin egg.
1998,
Pubmed
,
Echinobase
Covian-Nares,
Two independent forms of endocytosis maintain embryonic cell surface homeostasis during early development.
2008,
Pubmed
,
Echinobase
Dean,
Tumour stem cells and drug resistance.
2005,
Pubmed
Dean,
The human ATP-binding cassette (ABC) transporter superfamily.
2001,
Pubmed
Deeley,
Transmembrane transport of endo- and xenobiotics by mammalian ATP-binding cassette multidrug resistance proteins.
2006,
Pubmed
Duboc,
Left-right asymmetry in the sea urchin embryo is regulated by nodal signaling on the right side.
2005,
Pubmed
,
Echinobase
Eldakak,
Asymmetrically inherited multidrug resistance transporters are recessive determinants in cellular replicative ageing.
2010,
Pubmed
Essodaigui,
Kinetic analysis of calcein and calcein-acetoxymethylester efflux mediated by the multidrug resistance protein and P-glycoprotein.
1998,
Pubmed
Fletcher,
ABC transporters in cancer: more than just drug efflux pumps.
2010,
Pubmed
Fujii,
Role of the nanos homolog during sea urchin development.
2009,
Pubmed
,
Echinobase
Geisler,
The ABC of auxin transport: the role of p-glycoproteins in plant development.
2006,
Pubmed
Good,
Evidence that a cell-type-specific efflux pump regulates cell differentiation in Dictyostelium.
2000,
Pubmed
Good,
TagA, a putative serine protease/ABC transporter of Dictyostelium that is required for cell fate determination at the onset of development.
2003,
Pubmed
Gustafson,
Exogenous RNA is selectively retained in the small micromeres during sea urchin embryogenesis.
2010,
Pubmed
,
Echinobase
HORSTADIUS,
The mechanics of sea urchin development.
1950,
Pubmed
,
Echinobase
Hamdoun,
Activation of multidrug efflux transporter activity at fertilization in sea urchin embryos (Strongylocentrotus purpuratus).
2004,
Pubmed
,
Echinobase
Hara,
Exclusive expression of hedgehog in small micromere descendants during early embryogenesis in the sea urchin, Hemicentrotus pulcherrimus.
2005,
Pubmed
,
Echinobase
Harris,
Cdc42 and vesicle trafficking in polarized cells.
2010,
Pubmed
Juliano,
Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.
2006,
Pubmed
,
Echinobase
Juliano,
An evolutionary transition of Vasa regulation in echinoderms.
2009,
Pubmed
,
Echinobase
Juliano,
A conserved germline multipotency program.
2010,
Pubmed
,
Echinobase
Kim,
Experimentally induced changes in the endocytic traffic of P-glycoprotein alter drug resistance of cancer cells.
1997,
Pubmed
Klappe,
Lipid dependence of ABC transporter localization and function.
2009,
Pubmed
Kunwar,
In vivo migration: a germ cell perspective.
2006,
Pubmed
Lepage,
Expression of exogenous mRNAs to study gene function in the sea urchin embryo.
2004,
Pubmed
,
Echinobase
Logan,
Nuclear beta-catenin is required to specify vegetal cell fates in the sea urchin embryo.
1999,
Pubmed
,
Echinobase
Martys,
Wortmannin-sensitive trafficking pathways in Chinese hamster ovary cells. Differential effects on endocytosis and lysosomal sorting.
1996,
Pubmed
McClay,
Evolutionary crossroads in developmental biology: sea urchins.
2011,
Pubmed
,
Echinobase
McClay,
Sea urchin hyalin: appearance and function in development.
1982,
Pubmed
,
Echinobase
Oliveri,
Activation of pmar1 controls specification of micromeres in the sea urchin embryo.
2003,
Pubmed
,
Echinobase
Parton,
Ultrastructural localization of gangliosides; GM1 is concentrated in caveolae.
1994,
Pubmed
Pehrson,
The fate of the small micromeres in sea urchin development.
1986,
Pubmed
,
Echinobase
Ricardo,
An ABC transporter controls export of a Drosophila germ cell attractant.
2009,
Pubmed
Roepke,
Increase in multidrug transport activity is associated with oocyte maturation in sea stars.
2006,
Pubmed
,
Echinobase
Santos,
Germ cell specification and migration in Drosophila and beyond.
2004,
Pubmed
Schroeder,
Contact-independent polarization of the cell surface and cortex of free sea urchin blastomeres.
1988,
Pubmed
,
Echinobase
Shivas,
Polarity and endocytosis: reciprocal regulation.
2010,
Pubmed
Smith,
Testing the molecular clock: molecular and paleontological estimates of divergence times in the Echinoidea (Echinodermata).
2006,
Pubmed
,
Echinobase
Sparling,
Membrane fractions display different lipid and enzyme content in three cell types in 16-cell stage embryos of sea urchins.
1990,
Pubmed
,
Echinobase
Spiro,
Wortmannin alters the transferrin receptor endocytic pathway in vivo and in vitro.
1996,
Pubmed
Thorpe,
Germ cell migration in zebrafish is dependent on HMGCoA reductase activity and prenylation.
2004,
Pubmed
Uchida,
ABC transporter activities of murine hematopoietic stem cells vary according to their developmental and activation status.
2004,
Pubmed
Voronina,
Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development.
2008,
Pubmed
,
Echinobase
Wessel,
Isolation of organelles and components from sea urchin eggs and embryos.
2004,
Pubmed
,
Echinobase
Whalley,
Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.
1995,
Pubmed
,
Echinobase
Wu,
Isolation and characterization of Drosophila multidrug resistance gene homologs.
1991,
Pubmed
Yabe,
Multidrug resistance-associated protein MRP-1 regulates dauer diapause by its export activity in Caenorhabditis elegans.
2005,
Pubmed
Yajima,
Small micromeres contribute to the germline in the sea urchin.
2011,
Pubmed
,
Echinobase
Zhou,
Substrates and inhibitors of human multidrug resistance associated proteins and the implications in drug development.
2008,
Pubmed
Zhou,
Bcrp1 gene expression is required for normal numbers of side population stem cells in mice, and confers relative protection to mitoxantrone in hematopoietic cells in vivo.
2002,
Pubmed
