Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1987 May 01;849:2771-5. doi: 10.1073/pnas.84.9.2771.
Show Gene links
Show Anatomy links
Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.
Leslie RJ
,
Hird RB
,
Wilson L
,
McIntosh JR
,
Scholey JM
.
???displayArticle.abstract???
Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.
Bajer,
Role of phragmoplast filaments in cell-plate formation.
1966, Pubmed
Bajer,
Role of phragmoplast filaments in cell-plate formation.
1966,
Pubmed
Brady,
Fast axonal transport in extruded axoplasm from squid giant axon.
1982,
Pubmed
Branton,
Interaction of cytoskeletal proteins on the human erythrocyte membrane.
1981,
Pubmed
Cande,
In vitro reactivation of anaphase spindle elongation using isolated diatom spindles.
1985,
Pubmed
Euteneuer,
Structural polarity of kinetochore microtubules in PtK1 cells.
1981,
Pubmed
Fuge,
Ultrastructure of the mitotic spindle.
1977,
Pubmed
Harris,
The role of membranes in the ogranization of the mitotic apparatus.
1975,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Leslie,
Ultraviolet microbeam irradiations of mitotic diatoms: investigation of spindle elongation.
1983,
Pubmed
McDonald,
Cross-sectional structure of the central mitotic spindle of Diatoma vulgare. Evidence for specific interactions between antiparallel microtubules.
1979,
Pubmed
Mitchison,
Properties of the kinetochore in vitro. II. Microtubule capture and ATP-dependent translocation.
1985,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
OSTERGREN,
An interpretation of transport phenomena at mitosis.
1960,
Pubmed
Olmsted,
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
1981,
Pubmed
Pickett-Heaps,
Rethinking mitosis.
1982,
Pubmed
Rebhun,
Structural aspects of saltatory particle movement.
1967,
Pubmed
Rebhun,
Polarized intracellular particle transport: saltatory movements and cytoplasmic streaming.
1972,
Pubmed
Salmon,
Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).
1980,
Pubmed
,
Echinobase
Salmon,
Mitotic spindles isolated from sea urchin eggs with EGTA lysis buffers.
1982,
Pubmed
,
Echinobase
Scholey,
Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.
1986,
Pubmed
,
Echinobase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Vale,
Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon.
1985,
Pubmed
Vale,
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
1985,
Pubmed
Vale,
Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro.
1985,
Pubmed
Williams,
Preparation of tubulin from brain.
1982,
Pubmed
