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ECB-gRNA-26395076
gRNA Name:
LOC580458 gRNA3
Synonyms: Spmesh 450
Genomic Target: LOC580458
Sequence: 5' GGCTAAAGTCGACATTGCTC 3'
Synonyms: Spmesh 450
Genomic Target: LOC580458
Sequence: 5' GGCTAAAGTCGACATTGCTC 3'
| Genomic Target | S. purpuratus | A. planci | P. miniata | L. variegatus | |||
| Identities | 19/20 | ||||||
| genomic gRNA |
5' GAGCAATGTCGACTTTAGCG 3' ||||||||||||||||||| 3' CTCGTTACAGCTGAAATCGG 5' | ||||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | S. purpuratus 5.0 | NW_022145594.1:28423738..28423757 | 19/20 | 1 Antisense | target | LOC580458 |
| NW_022145615.1:32754741..32754760 | 17/20 | 2 Sense | off-target | mef2cl | |||
| NW_022145594.1:28279382..28279401 | 18/20 | 3 Antisense | off-target | LOC589327 | |||
| NW_022145594.1:28385902..28385921 | 18/20 | 4 Antisense | off-target | LOC586294 | |||
| NW_022145544.1:1839518..1839537 | 17/20 | 5 Antisense | off-target | LOC755676 | |||
| NW_022145612.1:44270441..44270460 | 17/20 | 6 Antisense | off-target | LOC115919281 | |||
| P. miniata 3.0 | NW_024037057.1:7905638..7905657 | 16/20 | 7 | unknown | |||
| L. variegatus 3.0 | chr1:10176425..10176444 | 18/20 | 8 Sense | off-target | LOC121425588 | ||
| chr12:7504605..7504624 | 16/20 | 9 Antisense | off-target | LOC121425559 | |||
| chr13:29494863..29494882 | 16/20 | 10 Antisense | off-target | LOC121426423 | |||
| Publications | |||||||
| First: |
Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022 |
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| Most recent: |
Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier., Dev Biol 2022 |
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| View All Papers | |||||||