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ECB-gRNA-26395073
gRNA Name:
LOC580458 gRNA1
Synonyms: Spmesh 306
Genomic Target: LOC580458
Sequence: 5' GGACGGGCGAATTGAGGACC 3'
Synonyms: Spmesh 306
Genomic Target: LOC580458
Sequence: 5' GGACGGGCGAATTGAGGACC 3'
| Genomic Target | S. purpuratus | A. planci | P. miniata | L. variegatus | |||
| Identities | 19/20 | ||||||
| genomic gRNA |
5' GGTCCTCAATTCGCCCGTCG 3' ||||||||||||||||||| 3' CCAGGAGTTAAGCGGGCAGG 5' | ||||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | S. purpuratus 5.0 | NW_022145594.1:28423591..28423610 | 19/20 | 1 Sense | target | LOC580458 |
| NW_022145594.1:28205545..28205564 | 19/20 | 2 Sense | off-target | LOC105439366 | |||
| NW_022145594.1:28239869..28239888 | 19/20 | 3 Sense | off-target | LOC115921309 | |||
| NW_022145594.1:28319664..28319683 | 18/20 | 4 Sense | off-target | LOC115918445 | |||
| NW_022145615.1:732201..732220 | 18/20 | 5 | unknown | ||||
| Publications | |||||||
| First: |
Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022 |
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| Most recent: |
Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier., Dev Biol 2022 |
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| View All Papers | |||||||