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Echinobase
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ECB-gRNA-26395062
gRNA Name: nanos2l gRNA4
Synonyms: SpNanos2 CKI1
Genomic Target: nanos2l

Sequence: 5' GGGTGTACGCCCTTAGTATC 3'



Genomic Target S. purpuratus A. planci P. miniata L. variegatus
Identities 19/20
genomic

gRNA		 5' GATACTAAGGGCGTACACCT 3' ||||||||||||||||||| 3' CTATGATTCCCGCATGTGGG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145605.1:14906213..14906232 19/20 1 Antisense target nanos2l
L. variegatus 3.0 chr2:69606340..69606359 18/20 2 Antisense off-target LOC121408828
Publications
First: Optimizing CRISPR/Cas9-based gene manipulation in echinoderms., Dev Biol 2022  
Most recent:
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