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Echinobase
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ECB-gRNA-26395058
gRNA Name: nodall gRNA5
Synonyms: E2_885
Genomic Target: nodall

Sequence: 5' GGTGTCCGTCTCTCGGGT 3'



Genomic Target S. purpuratus A. planci P. miniata L. variegatus
Identities 18/18
genomic

gRNA		 5' ACCCGAGAGACGGACACC 3' |||||||||||||||||| 3' TGGGCTCTCTGCCTGTGG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145595.1:2654986..2655003 18/18 1 Antisense target nodall
NW_022145603.1:18390929..18390946 17/18 2 Antisense off-target LOC754486
P. miniata 3.0 NW_024037072.1:32313715..32313732 16/18 3 Sense off-target LOC119741381
Publications
First: Genome editing in sea urchin embryos by using a CRISPR/Cas9 system., Dev Biol 2016  
Most recent:
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