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Echinobase

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ECB-GRNA-26395057
gRNA Name: nodall gRNA4
Synonyms: E2_777
Genomic Target: nodall
Derived from:

Sequence: 5' GGTAGGCGTTGAACTGCTTT 3'



Genomic Target S. purpuratus L. variegatus
Identities 20/20 20/20
genome
gRNA
 
5' AAAGCAGTTCAACGCCTACC 3'
   ||||||||||||||||||||
3' TTTCGTCAAGTTGCGGATGG 5'
 
5' AAAGCAGTTCAACGCCTACC 3'
   ||||||||||||||||||||
3' TTTCGTCAAGTTGCGGATGG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145595.1:2654878..2654897 20/20 Antisense target nodall
NW_022145600.1:26255920..26255939 18/20 Sense off-target LOC105442316
P. miniata 3.0 NW_024037061.1:15644621..15644640 18/20 Antisense off-target LOC119730627
L. variegatus 3.0 chr18:12696838..12696857 20/20 Antisense target nodall
chr8:9818816..9818835 16/20 Sense off-target LOC121419950
chr6:406069..406088 16/20 Sense off-target LOC121416721
chr13:19161343..19161362 18/20 unknown
Publications
???morpholino.displaySummary.papersFirst??? Genome editing in sea urchin embryos by using a CRISPR/Cas9 system., Dev Biol 2016  
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