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ECB-gRNA-26395057
gRNA Name:
nodall gRNA4
Synonyms: E2_777
Genomic Target: nodall
Sequence: 5' GGTAGGCGTTGAACTGCTTT 3'
Synonyms: E2_777
Genomic Target: nodall
Sequence: 5' GGTAGGCGTTGAACTGCTTT 3'
| Genomic Target | S. purpuratus | A. planci | P. miniata | L. variegatus | |||
| Identities | 20/20 | ||||||
| genomic gRNA |
5' AAAGCAGTTCAACGCCTACC 3' |||||||||||||||||||| 3' TTTCGTCAAGTTGCGGATGG 5' | ||||||
Genomic Alignments  |
Position | Identity | Strand | Target | Genomic Target | S. purpuratus 5.0 | NW_022145595.1:2654878..2654897 | 20/20 | 1 Antisense | target | nodall |
| NW_022145600.1:26255920..26255939 | 18/20 | 2 Sense | off-target | LOC105442316 | |||
| P. miniata 3.0 | NW_024037061.1:15644621..15644640 | 18/20 | 3 Antisense | off-target | LOC119730627 | ||
| L. variegatus 3.0 | chr18:12696838..12696857 | 20/20 | 4 Antisense | target | nodall | ||
| chr6:406069..406088 | 16/20 | 5 Sense | off-target | LOC121416721 | |||
| chr8:9818816..9818835 | 16/20 | 6 Sense | off-target | LOC121419950 | |||
| chr13:19161343..19161362 | 18/20 | 7 | unknown | ||||
| Publications | |||||||
| First: |
Genome editing in sea urchin embryos by using a CRISPR/Cas9 system., Dev Biol 2016 |
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| Most recent: | |||||||
| View All Papers | |||||||