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Echinobase

Summary Attributions Wiki
ECB-GRNA-26395055
gRNA Name: nodall gRNA2
Synonyms: E1_272
Genomic Target: nodall
Derived from:

Sequence: 5' GGAAGAATGCCAAGCCATTG 3'



Genomic Target S. purpuratus
Identities 20/20
genome
gRNA
 
5' CAATGGCTTGGCATTCTTCC 3'
   ||||||||||||||||||||
3' GTTACCGAACCGTAAGAAGG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145595.1:2652723..2652742 20/20 Sense target nodall
NW_022145607.1:40693278..40693297 18/20 Antisense off-target dmd
NW_022145607.1:22432803..22432822 18/20 Sense off-target LOC100889296
NW_022145610.1:16197043..16197062 18/20 Antisense off-target LOC105442958
NW_022145615.1:34342443..34342462 18/20 Antisense off-target LOC115920546
NW_022145599.1:35232763..35232782 18/20 Sense unknown
NW_022145603.1:32392362..32392381 18/20 Antisense unknown
NW_022145606.1:22513083..22513102 18/20 Sense unknown
NW_022145615.1:24425785..24425804 18/20 Sense unknown
NW_022145609.1:20070681..20070700 17/20 Antisense unknown
NW_022145600.1:3693184..3693203 16/20 Antisense unknown
P. miniata 3.0 NW_024037049.1:7329174..7329193 17/20 Antisense off-target LOC119743429
NW_024037049.1:7446150..7446169 17/20 Antisense off-target LOC119743444
NW_024037056.1:10657973..10657992 17/20 Sense off-target LOC119724565
XR_005270983.1:5569..5588 17/20 off-target LOC119727544
NW_024037047.1:27205692..27205711 16/20 Sense off-target LOC119727353
NW_024037060.1:3198968..3198987 16/20 Sense off-target LOC119729899
NW_024037055.1:111158..111177 17/20 Antisense unknown
NW_024037047.1:11701052..11701071 16/20 Antisense unknown
NW_024037047.1:3692554..3692573 16/20 Sense unknown
NW_024037073.1:16935378..16935397 16/20 Antisense unknown
L. variegatus 3.0 chr3:30644733..30644752 18/20 Sense off-target LOC121410751
chr6:39134411..39134430 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Genome editing in sea urchin embryos by using a CRISPR/Cas9 system., Dev Biol 2016  
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