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Echinobase
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ECB-gRNA-26395015
gRNA Name: nodall gRNA1
Synonyms: E1_157
Genomic Target: nodall

Sequence: 5' GGGCGTCCGGTGTGATAAG 3'



Genomic Target S. purpuratus A. planci P. miniata L. variegatus
Identities 19/19
genomic

gRNA		 5' CTTATCACACCGGACGCCC 3' ||||||||||||||||||| 3' GAATAGTGTGGCCTGCGGG 5'
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145595.1:2652607..2652625 19/19 1 Antisense target nodall
NW_022145599.1:10857151..10857169 15/19 2 unknown
NW_022145599.1:11041593..11041611 15/19 3 unknown
P. miniata 3.0 NW_024037074.1:35314432..35314450 17/19 4 Antisense off-target LOC119745886
NW_024037047.1:34505095..34505113 15/19 5 Sense off-target LOC119729096
NW_024037047.1:35105390..35105408 15/19 6 Sense off-target LOC119722451
Publications
First: Genome editing in sea urchin embryos by using a CRISPR/Cas9 system., Dev Biol 2016  
Most recent: CRISPR/Cas9 mutagenesis reveals a role for ABCB1 in gut immune responses to Vibrio diazotrophicus in sea urchin larvae., J Exp Biol 2021  
View All Papers