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Echinobase

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ECB-ANTIBODY-26396052

Antibody Name: emap Ab2

Common Name: 77 kD echinoderm microtubule-associated protein

RRID:
Synonyms: 77kDa-EMAP, A58, major microtubule-associated protein

Antigens
Gene: emap

Clone Type: Polyclonal
Source:

Antibody
Clone Number
Purification Affinity Purification
Isotype IgG
Host Organism rabbit
Echinoderm Reactivity S. purpuratus, L. pictus
Non-Echinoderm Reactivity cow
Description: Reacts with mitotic apparatuses in dividing embryonic and somatic cells of various echinoderms, including sea urchins (Arbacia punctulata, Lytechinus variegatus), sand dollars (Echinarachnius parma), and sea stars (Asterias vulgaris). Created by the Suprenant laboratory at the University of Kansas. Also reacts with Spodoptera frugiperda (fall armyworm).
Immunogen
Name 77,000 MW Polypeptide
Type polypeptide
Post Translational Modifications None
Source Organism purple sea urchin
Description Protein excised and electroeluted from SDS-polyacrylamide gel that was utilized to separate cycle-purified microtubules originating from sea urchin eggs.
Sequence
Reported Usage
immunofluorescence
immunoprecipitation
western blot
Publications
???displayAntibody.papersFirst??? EMAP, an echinoderm microtubule-associated protein found in microtubule-ribosome complexes., J Cell Sci 1993  
???displayAntibody.papersRecent??? Saturable binding of the echinoderm microtubule-associated protein (EMAP) on microtubules, but not filamentous actin or vimentin filaments., Cell Motil Cytoskeleton 2001  
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