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Echinobase

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ECB-ANTIBODY-26396041

Antibody Name: tubb1 Ab6

Common Name: tubulin beta 1 class 6

RRID: AB_372176
Synonyms: D66, beta-tubulin

Antigens
Gene: tubb1

Clone Type: Monoclonal
Source: GeneTex, Novus Biologicals, Sigma-Aldrich

Antibody
Clone Number D66
Purification Affinity Purification
Isotype IgG1
Host Organism mouse
Echinoderm Reactivity S. purpuratus, L. pictus
Non-Echinoderm Reactivity common urchin, cow, dog, human, monkey, mouse, rabbit, rat
Description: Antibody demonstrated to inhibit sea urchin sperm mobility by recognizing a carboxy-terminal epitope (Asp427-Glu352) of beta-tubulin. Created and screened from a panel of monoclonal antibodies by the Gagnon lab (Urology Research Laboratory, Royal Victoria Hospital, McGill University, Montreal, Canada). Currently supplied by GeneTex (#GTX11307), Novus Biologicals (#NB600-1514), and Sigma-Aldrich (#T0198).
Immunogen
Name Sperm Axoneme Proteins
Type polypeptide
Post Translational Modifications None
Source Organism Lytechinus pictus
Description Purified extract originating from sea urchin spermatozoa.
Sequence
Reported Usage
enzyme-linked immunosorbent assay
immunofluorescence
immunoprecipitation
western blot
Publications
???displayAntibody.papersFirst??? Molecular cloning and characterization of a radial spoke head protein of sea urchin sperm axonemes: involvement of the protein in the regulation of sperm motility., Mol Biol Cell 1998  
???displayAntibody.papersRecent??? Protein kinase C is an important signaling mediator associated with motility of intact sea urchin spermatozoa., J Exp Biol 2007  
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