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Echinobase

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ECB-ANTIBODY-26396032

Antibody Name: emap Ab1

Common Name: 77 kD echinoderm microtubule-associated protein

RRID:
Synonyms: 77kDa-MAP, Major Microtubule-Associated Protein, S.p. 77-2

Antigens
Gene: emap

Clone Type: Monoclonal
Source:

Antibody
Clone Number S.p. 77-2
Purification
Isotype IgG1
Host Organism mouse
Echinoderm Reactivity S. purpuratus, L. pictus
Non-Echinoderm Reactivity
Description: Monospecific antibody that reacts with mitotic spindle apparatuses and phagocytic amoebocytes in sea urchins. Created and previously supplied by the lab of R. Vallee (Worcester Foundation, Worcester, MA). Also reacts with Lytechinus variegatus, Arbacia punctulata, and Asterias vulgaris.
Immunogen
Name 77,000-Dalton polypeptide
Type polypeptide
Post Translational Modifications None
Source Organism purple sea urchin
Description Protein electroeluted from SDS-polyacrylamide gel utilized to separate cycle-purified microtubule proteins isolated from sea urchin eggs.
Sequence
Reported Usage
immunofluorescence
western blot
Publications
???displayAntibody.papersFirst??? Use of multiple monoclonal antibodies to characterize the major microtubule-associated protein in sea urchin eggs., Cell Motil 1985  
???displayAntibody.papersRecent??? EMAP, an echinoderm microtubule-associated protein found in microtubule-ribosome complexes., J Cell Sci 1993  
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