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Echinobase

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ECB-ANTIBODY-26395877

Antibody Name: tbxt Ab1

Common Name: T-box transcription factor T

RRID:
Synonyms: Brachyury, SpBra

Antigens
Gene: tbxt

Clone Type: Polyclonal
Source:

Antibody
Clone Number
Purification Affinity Purification
Isotype isotype unknown
Host Organism rabbit
Echinoderm Reactivity S. purpuratus
Non-Echinoderm Reactivity
Description: Specifically reacts with a ~50 kDa protein located in the endoderm surrounding the blastopore in sea urchin gastrulae. Antibody generated and affinity-purified by PRIMM Biotech for usage by the laboratories of Maria Ina Arnone (Stazione Zoologica Anton Dohrn, Villa Comunale, Naples, Italy) and Ulrich Technau (Max Perutz Labs, University of Vienna, Vienna, Austria).
Immunogen
Name Brachyury Protein
Type polypeptide
Post Translational Modifications None
Source Organism purple sea urchin
Description Recombinantly produced protein containing the C-terminal domain, excluding the T-box domain.
Sequence
Reported Usage
chromatin immunoprecipitation
immunofluorescence
immunohistochemistry
western blot
Publications
???displayAntibody.papersFirst??? An ancestral Wnt-Brachyury feedback loop in axial patterning and recruitment of mesoderm-determining target genes., Nat Ecol Evol 2022  
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