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Echinobase

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ECB-ANTIBODY-26395580

Antibody Name: kif23 Ab1

Common Name: kinesin family member 23

RRID:
Synonyms: 110-kD kinesin-like protein, CHO1, KRP110

Antigens
Gene: kif23

Clone Type: Monoclonal
Source:

Antibody
Clone Number CHO1
Purification Ascites
Isotype IgM
Host Organism mouse
Echinoderm Reactivity L. variegatus, S. purpuratus, L. pictus
Non-Echinoderm Reactivity human
Description: Antibody demonstrated to arrest mitosis and prevent cell division in sea urchin embryos by targeting a mitotic spindle protein. Originally created and previously supplied by the laboratory of R. Kuriyama (University of Minnesota, Minneapolis, MN) (PMID: 2448315). Has also been supplied by J.R. McIntosh and C. Nislow (University of Colorado, Boulder, CO) (PMID: 2199459).
Immunogen
Name CHO Mitotic Spindle Fraction
Type polypeptide
Post Translational Modifications None
Source Organism Other
Description Midbody microtubule extract purified from mitotic chinese hamster ovary cells arrested at S phase using taxol-containing medium.
Sequence
Reported Usage
immunofluorescence
western blot
Publications
???displayAntibody.papersFirst??? Roles of kinesin and kinesin-like proteins in sea urchin embryonic cell division: evaluation using antibody microinjection., J Cell Biol 1993  
???displayAntibody.papersRecent??? Roles of two homotetrameric kinesins in sea urchin embryonic cell division., J Biol Chem 2000  
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