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Echinobase

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ECB-ANTIBODY-26395562

Antibody Name: LOC373178 Ab5

Common Name: kinesin heavy chain

RRID:
Synonyms: SUK5, kinesin family member 5B

Antigens
Gene: LOC373178

Clone Type: Monoclonal
Source:

Antibody
Clone Number SUK5
Purification Affinity Purification
Isotype IgG2b
Host Organism mouse
Echinoderm Reactivity S. purpuratus, L. pictus
Non-Echinoderm Reactivity cow
Description: Antibody that cross-reacts with a 120-kD protein in bovine brain and specifically binds to the tail region (amino acids 859-1031) of a membrane-bound protein in microtubules of mitotic embryos. Created by Amie L. Ingold, Stanley A. Cohn, and Jonathan M. Scholey at the University of Colorado Health Science Center (PMID: 2974459) and characterized by the Scholey lab at the University of California in Davis (PMID: 1827446).
Immunogen
Name ATP/EDTA Recycled Kinesin
Type polypeptide
Post Translational Modifications None
Source Organism purple sea urchin
Description Native 130-kD protein affinity-purified from microtubules extracted from sea urchin eggs.
Sequence
Reported Usage
immunohistochemistry
western blot
immunoprecipitation
immunofluorescence
enzyme-linked immunosorbent assay
Publications
???displayAntibody.papersFirst??? Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains., J Cell Biol 1988  
???displayAntibody.papersRecent??? Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis., J Cell Biol 1997              
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