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Echinobase

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ECB-ANTIBODY-26395423

Antibody Name: nrf1 Ab2

Common Name: nuclear respiratory factor 1

RRID:
Synonyms: 7B12-2E8, DNA-binding protein P3A2, SpP3A2

Antigens
Gene: nrf1

Clone Type: Monoclonal
Source:

Antibody
Clone Number 7B12-2E8
Purification
Isotype isotype unknown
Host Organism mouse
Echinoderm Reactivity S. purpuratus
Non-Echinoderm Reactivity
Description: Inhibits DNA-binding and subsequent restricted aboral expression of CyIIIa in the cytoskeleton. Created in the Caltech Monoclonal Antibody Facility for usage in the Davidson lab at the California Institute of Technology.
Immunogen
Name Recombinant P3A2
Type polypeptide
Post Translational Modifications None
Source Organism purple sea urchin
Description Full-length recombinant protein affinity-purified from bacterial cultures using six-histidine tag.
Sequence
Reported Usage
electrophoretic mobility shift assay
western blot
Publications
???displayAntibody.papersFirst??? Developmental utilization of SpP3A1 and SpP3A2: two proteins which recognize the same DNA target site in several sea urchin gene regulatory regions., Dev Biol 1995  
???displayAntibody.papersRecent??? Interference with gene regulation in living sea urchin embryos: transcription factor knock out (TKO), a genetically controlled vector for blockade of specific transcription factors., Proc Natl Acad Sci U S A 1998  
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