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ECB-ANTIBODY-26395341

Antibody Name: LOC100888451 Ab1

Common Name: uncharacterized LOC100888451

RRID:
Synonyms: 3G9, Sp-Fsialin, flagellasialin, polySia-containing glycoprotein of sperm flagella

Antigens
Gene: LOC100888451

Clone Type: Monoclonal
Source:

Antibody
Clone Number 3G9
Purification
Isotype IgM
Host Organism mouse
Echinoderm Reactivity S. purpuratus, H. pulcherrimus
Non-Echinoderm Reactivity
Description: Recognizes a major sulfated ganglioside epitope, 8-Osulfated Neu5Ac, located on several sperm glycoproteins, one of which includes a 40-80 kDa protein (flagellasialin). Created in the Kitajima lab at Nagoya University in Japan. Also reacts with Anthocidaris crassispina and Strongyocentrotus franciscanus.
Immunogen
Name Acrosome Reacted Sperm
Type other immunogen type
Post Translational Modifications Glycosylation
Source Organism Hemicentrotus pulcherrimus
Description Sperm suspension supplemented with the ganglioside, HSO3(Neu5Ac)2GlcC.
Sequence
Reported Usage
enzyme-linked immunosorbent assay
western blot
Publications
???displayAntibody.papersFirst??? Isolation and characterization of low density detergent-insoluble membrane (LD-DIM) fraction from sea urchin sperm., Biochem Biophys Res Commun 1999  
???displayAntibody.papersRecent??? Mechanism regulating Ca2+-dependent mechanosensory behaviour in sea urchin spermatozoa., Cell Struct Funct 2011  
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