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Fish Shellfish Immunol Rep
2022 Dec 29;4:100074. doi: 10.1016/j.fsirep.2022.100074.
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Comparative analysis for immune response of coelomic fluid from coelom and polian vesicle in Apostichopus japonicus to Vibrio splendidus infection.
Wang Z
,
Fan X
,
Li Z
,
Guo L
,
Ren Y
,
Li Q
.
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The polian vesicle and coelom of sea cucumber Apostichopus japonicus were full of coelomic fluid in which many types of coelomocytes with different functions were suspended. Our previous work has indicated the differences of coelomocytes between two sites mainly in subtype proportion, non-specific immune enzymes activities and several immune-related genes expression levels in healthy A. japonicus. However, the functional similarities and differences of coelomic fluid in two sites including the coelom and polian vesicle after pathogenic infection still remain unclear. Here, we investigated the changes of the total coelomocyte density (TCD) and differential coelomocyte density (DCD) after pathogen infection by Vibrio splendidus in coelom and polian vesicle. After infected by V. splendidus, the TCD in the coelom and polian vesicle rapidly declined at 12 h, and then the TCD in the coelom showed a stably ascending trend, while the TCD in the polian vesicle reached a peak at 24 h post infection (hpi), and then showed a continuously decline trend from 24 hpi to 72 hpi followed by a slow elevation until recovering the normal level from 72 hpi to 96 hpi. Then the activities of acidic phosphatase (ACP), alkaline phosphatase (AKP), catalase (CAT) and superoxide dismutase (SOD) were determined to evaluate the response of cell-free coelomic fluid to V. splendidus infection. The activities of ACP, AKP and CAT showed similar trends in the coelom and polian vesicle. The SOD activity significantly increased in the polian vesicle, whereas it exhibited a decreasing trend in the coelom. Finally, the expression profiles of nine immune-related genes including Aj-MyD88, Aj-IRAK4, Aj-i-Lys, Aj-Rel, Aj-p50, Aj-DMBT1, Aj-CDC, Aj-Rrp15 and Aj-Fibrinogen C were detected after V. splendidus challenge. The results suggested all the detected genes were significantly up-regulated both in the coelom and polian vesicle, and the expression levels of these genes in two sites shared similar trends except Aj-MyD88 and Aj-DMBT1. This research provides a new insight into the differentially immune roles of coelomic fluid and coelomocytes in polian vesicle and coelom response to bacterial infections and supplements comprehensive resources for better understanding the innate immune response of A. japonicus.
Baccetti,
The fine structure of polian vesicles of holothurians.
1968, Pubmed,
Echinobase
Baccetti,
The fine structure of polian vesicles of holothurians.
1968,
Pubmed
,
Echinobase
Balboa,
The role of lipins in innate immunity and inflammation.
2019,
Pubmed
Bertheussen,
Endocytosis by echinoid phagocytes in vitro. II. Mechanisms of endocytosis.
1981,
Pubmed
,
Echinobase
D'Ancona,
Engagement of Polian vesicles during Holothuria polii response to erythrocyte injection.
1989,
Pubmed
,
Echinobase
Dong,
Expression analysis of immune related genes identified from the coelomocytes of sea cucumber (Apostichopus japonicus) in response to LPS challenge.
2014,
Pubmed
,
Echinobase
Dybas,
Holothurian survival strategies: mechanisms for the maintenance of a bacteriostatic environment in the coelomic cavity of the sea cucumber, Parastichopus californicus.
1986,
Pubmed
,
Echinobase
Gao,
Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge.
2015,
Pubmed
,
Echinobase
Giannapas,
Generation of free radicals in haemocytes of mussels after exposure to low molecular weight PAH components: immune activation, oxidative and genotoxic effects.
2012,
Pubmed
Gliński,
Immune phenomena in echinoderms.
2000,
Pubmed
,
Echinobase
Goyal,
Human catalase: looking for complete identity.
2010,
Pubmed
Guo,
Transcriptome analysis reveals roles of polian vesicle in sea cucumber Apostichopus japonicus response to Vibrio splendidus infection.
2021,
Pubmed
,
Echinobase
Johnson,
The coelomic elements of sea urchins (Strongylocentrotus). 3. In vitro reaction to bacteria.
1969,
Pubmed
,
Echinobase
Kozloff,
Wahlia pulchella n. sp., a turbellarian flatworm (Neorhabdocoela: Umagillidae) from the intestine of the sea cucumber Stichopus californicus.
1987,
Pubmed
,
Echinobase
Li,
Comparison of cells free in coelomic and water-vascular system of sea cucumber, Apostichopus japonicus.
2013,
Pubmed
,
Echinobase
Li,
Regeneration of coelomocytes after evisceration in the sea cucumber, Apostichopus japonicus.
2018,
Pubmed
,
Echinobase
Li,
Localization and characterization of hematopoietic tissues in adult sea cucumber, Apostichopus japonicus.
2019,
Pubmed
,
Echinobase
Liu,
Identification of the pathogens associated with skin ulceration and peristome tumescence in cultured sea cucumbers Apostichopus japonicus (Selenka).
2010,
Pubmed
,
Echinobase
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Maeda,
Innate immunity in allergy.
2019,
Pubmed
McCord,
SOD, oxidative stress and human pathologies: a brief history and a future vision.
2005,
Pubmed
Nozik-Grayck,
Extracellular superoxide dismutase.
2005,
Pubmed
Panda,
Innate Lymphoid Cells in Mucosal Immunity.
2019,
Pubmed
Płytycz,
Bacterial clearance by the sea urchin, Strongylocentrotus droebachiensis.
1993,
Pubmed
,
Echinobase
Rader,
Alkaline Phosphatase, an Unconventional Immune Protein.
2017,
Pubmed
Reinisch,
Cell recognition: reactions of the sea star (Asterias vulgaris) to the injection of amebocytes of sea urchin (Arbacia punctulata).
1971,
Pubmed
,
Echinobase
Ren,
Non-specific immune factors differences in coelomic fluid from polian vesicle and coelom of Apostichopus japonicus, and their early response after evisceration.
2020,
Pubmed
,
Echinobase
Schmid-Hempel,
Variation in immune defence as a question of evolutionary ecology.
2003,
Pubmed
Singh,
Role of Intestinal Alkaline Phosphatase in Innate Immunity.
2021,
Pubmed
Smith,
Echinoderm immunity.
2010,
Pubmed
,
Echinobase
Smith,
A proposed phylogenetic relationship between sea cucumber polian vesicles and the vertebrate lymphoreticular system.
1978,
Pubmed
,
Echinobase
Wang,
First Report on Natural Infection of Nodavirus in an Echinodermata, Sea Cucumber (Apostichopus japonicas).
2021,
Pubmed
,
Echinobase
Xue,
A review of the immune molecules in the sea cucumber.
2015,
Pubmed
,
Echinobase
Yui,
ECHINODERM IMMUNOLOGY: BACTERIAL CLEARANCE BY THE SEA URCHIN STRONGYLOCENTROTUS PURPURATUS.
1983,
Pubmed
,
Echinobase
Zelko,
Superoxide dismutase multigene family: a comparison of the CuZn-SOD (SOD1), Mn-SOD (SOD2), and EC-SOD (SOD3) gene structures, evolution, and expression.
2002,
Pubmed
Zhang,
Effects of l-tryptophan on the growth, intestinal enzyme activities and non-specific immune response of sea cucumber (Apostichopus japonicus Selenka) exposed to crowding stress.
2018,
Pubmed
,
Echinobase
Zhang,
Molecular cloning and functional characterization of MYC transcription factor in pathogen-challenged Apostichopus japonicus.
2020,
Pubmed
,
Echinobase
Zhang,
Proteomic identification of differentially expressed proteins in sea cucumber Apostichopus japonicus coelomocytes after Vibrio splendidus infection.
2014,
Pubmed
,
Echinobase
Zhuang,
Cloning and characterization of the virulence factor Hop from Vibrio splendidus.
2020,
Pubmed
,
Echinobase
