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Cell Tissue Res
2023 Mar 01;3913:441-456. doi: 10.1007/s00441-023-03738-w.
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Immunohistochemical localisation of vasopressin/oxytocin-type, corazonin-type and luqin-type neuropeptide expression in the starfish Asterias rubens using antibodies to the C-terminal region of precursor proteins.
Tinoco AB
,
Egertová M
,
Elphick MR
.
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Neuropeptides derived from larger precursor proteins are secreted as signalling molecules by neurons and regulate diverse physiological and behavioural processes in animals. Recently, we reported the discovery of ArCRZ (HNTFTMGGQNRWKAG-NH2) and ArLQ (EEKTRFPKFMRW-NH2)-novel neuropeptides in the starfish Asterias rubens that are orthologs of arthropod corazonins and molluscan luqins, respectively. However, our efforts to generate antibodies to ArCRZ and ArLQ have been unsuccessful, precluding immunohistochemical analysis of their expression. Here, we investigated an alternative experimental approach for neuropeptide immunohistochemistry by generating antibodies to peptides corresponding to the C-terminal region of the precursor proteins. As proof of principle, we generated antibodies to the C-terminal region of the precursor of the vasopressin/oxytocin-type neuropeptide asterotocin and show that these reveal immunostaining in A. rubens that is very similar to that observed with asterotocin antibodies. Furthermore, antibodies to the C-terminal region of the ArCRZ precursor (ArCRZP) and the ArLQ precursor (ArLQP) produced patterns of immunostaining consistent, respectively, with the distribution of ArCRZP and ArLQP transcripts revealed by mRNA in situ hybridisation. Detailed immunohistochemical analysis revealed widespread expression of ArCRZP and ArLQP in A. rubens, including the central nervous system, digestive system and the body wall and its associated appendages (e.g. tube feet), providing a neuroanatomical framework for investigation and interpretation of the pharmacological actions of ArCRZ and ArLQ in A. rubens. Furthermore, our findings provide a basis for use of antibodies to the C-terminal region of neuropeptide precursor proteins in other species where the production of antibodies to the bioactive neuropeptides is unsuccessful.
Aloyz,
Processing of the L5-67 precursor peptide and characterization of LUQIN in the LUQ neurons of Aplysia californica.
1995, Pubmed
Aloyz,
Processing of the L5-67 precursor peptide and characterization of LUQIN in the LUQ neurons of Aplysia californica.
1995,
Pubmed
Blowes,
Body wall structure in the starfish Asterias rubens.
2017,
Pubmed
,
Echinobase
Cai,
Biochemical, Anatomical, and Pharmacological Characterization of Calcitonin-Type Neuropeptides in Starfish: Discovery of an Ancient Role as Muscle Relaxants.
2018,
Pubmed
,
Echinobase
Cai,
Molecular Identification and Cellular Localization of a Corticotropin-Releasing Hormone-Type Neuropeptide in an Echinoderm.
2023,
Pubmed
,
Echinobase
Cazzamali,
Molecular cloning and functional expression of a Drosophila corazonin receptor.
2002,
Pubmed
Dockray,
The biosynthesis of regulatory peptides.
1987,
Pubmed
Elphick,
Evolution of neuropeptide signalling systems.
2018,
Pubmed
Fujimoto,
A novel cardio-excitatory peptide isolated from the atria of the African giant snail, Achatina fulica.
1990,
Pubmed
Hauser,
Evolution of the AKH/corazonin/ACP/GnRH receptor superfamily and their ligands in the Protostomia.
2014,
Pubmed
Jékely,
Global view of the evolution and diversity of metazoan neuropeptide signaling.
2013,
Pubmed
Jékely,
The long and the short of it - a perspective on peptidergic regulation of circuits and behaviour.
2018,
Pubmed
Larsen,
Somatostatin in the retina.
1995,
Pubmed
Larsen,
Somatostatin and prosomatostatin in the retina of the rat: an immunohistochemical, in-situ hybridization, and chromatographic study.
1990,
Pubmed
Li,
Molecular and functional characterization of the luqin-type neuropeptide signaling system in the sea cucumber Apostichopus japonicus.
2022,
Pubmed
,
Echinobase
Lin,
Pedal peptide/orcokinin-type neuropeptide signaling in a deuterostome: The anatomy and pharmacology of starfish myorelaxant peptide in Asterias rubens.
2017,
Pubmed
,
Echinobase
Lin,
Functional characterization of a second pedal peptide/orcokinin-type neuropeptide signaling system in the starfish Asterias rubens.
2018,
Pubmed
,
Echinobase
Melarange,
Comparative analysis of nitric oxide and SALMFamide neuropeptides as general muscle relaxants in starfish.
2003,
Pubmed
,
Echinobase
Mirabeau,
Molecular evolution of peptidergic signaling systems in bilaterians.
2013,
Pubmed
Odekunle,
Ancient role of vasopressin/oxytocin-type neuropeptides as regulators of feeding revealed in an echinoderm.
2019,
Pubmed
,
Echinobase
Ohno,
Luqin-like RYamide peptides regulate food-evoked responses in C. elegans.
2017,
Pubmed
Park,
Identification of G protein-coupled receptors for Drosophila PRXamide peptides, CCAP, corazonin, and AKH supports a theory of ligand-receptor coevolution.
2002,
Pubmed
Semmens,
Transcriptomic identification of starfish neuropeptide precursors yields new insights into neuropeptide evolution.
2016,
Pubmed
,
Echinobase
Shyamala,
A neuropeptide precursor expressed in Aplysia neuron L5.
1986,
Pubmed
Tensen,
The lymnaea cardioexcitatory peptide (LyCEP) receptor: a G-protein-coupled receptor for a novel member of the RFamide neuropeptide family.
1998,
Pubmed
Tian,
Urbilaterian origin of paralogous GnRH and corazonin neuropeptide signalling pathways.
2016,
Pubmed
,
Echinobase
Tian,
Functional Characterization of Paralogous Gonadotropin-Releasing Hormone-Type and Corazonin-Type Neuropeptides in an Echinoderm.
2017,
Pubmed
,
Echinobase
Tinoco,
Ancient role of sulfakinin/cholecystokinin-type signalling in inhibitory regulation of feeding processes revealed in an echinoderm.
2021,
Pubmed
,
Echinobase
Veenstra,
Isolation and structure of corazonin, a cardioactive peptide from the American cockroach.
1989,
Pubmed
Yañez-Guerra,
Evolution and Comparative Physiology of Luqin-Type Neuropeptide Signaling.
2020,
Pubmed
,
Echinobase
Yañez-Guerra,
Discovery and functional characterisation of a luqin-type neuropeptide signalling system in a deuterostome.
2018,
Pubmed
,
Echinobase
Zandawala,
The evolution and nomenclature of GnRH-type and corazonin-type neuropeptide signaling systems.
2018,
Pubmed
,
Echinobase
Zhang,
Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate.
2020,
Pubmed
,
Echinobase
Zhang,
Somatostatin-type and allatostatin-C-type neuropeptides are paralogous and have opposing myoregulatory roles in an echinoderm.
2022,
Pubmed
,
Echinobase
van Minnen,
Stimulus-dependent translocation of egg-laying hormone encoding mRNA into the axonal compartment of the neuroendocrine caudodorsal cells.
2003,
Pubmed