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ECB-ART-51009
Carbohydr Polym October 15, 2022; 294 119826.
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Re-understanding of structure and anticoagulation: Fucosylated chondroitin sulfate from sea cucumber Ludwigothurea grisea.

Yin R , Pan Y , Cai Y , Yang F , Gao N , Ruzemaimaiti D , Zhao J .


Abstract
Fucosylated chondroitin sulfate (FCS) from sea cucumber Ludwigothurea grisea (FCSLg) is the first one that reported to bear the di-fucosyl branches. Here we deciphered it by analyzing the physicochemical properties and its derivatives. Oligosaccharides prepared by selective cleavage of glycosidic linkages presented the mono-fucose and heterodisaccharide branches in FCSLg. The disaccharide branch was determined as d-GalNAcR1-(α1,2)-l-FucR2 rather than the di-fucosyl branch, where R1 was 4-mono-O- or 4,6-di-O-sulfation, and R2 was 3-mono-O- or 3,4-di-O-sulfation, respectively. The diversity of sulfation patterns in branches complicated the structure. These results give us a new understanding of FCSLg and provided a reliable method to decipher the FCS with complex branches. Bioanalysis of chemically modified derivatives showed that modulating the molecular mass could enhance the Xase target selectivity. Side chains conferred the Xase complex inhibition by binding to FIXa with a high affinity. Whether monosaccharide and disaccharide branches have differential effects needs to be further explored.

PubMed ID: 35868774
Article link: Carbohydr Polym