ECB-ART-50982Dev Biol October 1, 2022; 490 117-124.
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Optimizing CRISPR/Cas9-based gene manipulation in echinoderms.
The impact of new technology can be appreciated by how broadly it is used. Investigators that previously relied only on pharmacological approaches or the use of morpholino antisense oligonucleotide (MASO) technologies are now able to apply CRISPR-Cas9 to study biological problems in their model organism of choice much more effectively. The transitions to new CRISPR-based approaches could be enhanced, first, by standardized protocols and education in their applications. Here we summarize our results for optimizing the CRISPR-Cas9 technology in a sea urchin and a sea star, and provide advice on how to set up CRISPR-Cas9 experiments and interpret the results in echinoderms. Our goal through these protocols and sharing examples of success by other labs is to lower the activation barrier so that more laboratories can apply CRISPR-Cas9 technologies in these important animals.
PubMed ID: 35917936
Article link: Dev Biol
gRNAs referenced: LOC580458 gRNA1 LOC580458 gRNA2 LOC580458 gRNA3 LOC584189 gRNA3 LOC584189 gRNA4 LOC584189 gRNA5 LOC586240 gRNA1 LOC588806 gRNA20 LOC588806 gRNA21 foxy gRNA1 foxy gRNA2 foxy gRNA3 nanos2l gRNA4