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Proc Natl Acad Sci U S A
2022 Oct 04;11940:e2203904119. doi: 10.1073/pnas.2203904119.
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Extracellular carbonic anhydrase activity promotes a carbon concentration mechanism in metazoan calcifying cells.
Matt AS
,
Chang WW
,
Hu MY
.
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Many calcifying organisms utilize metabolic CO2 to generate CaCO3 minerals to harden their shells and skeletons. Carbonic anhydrases are evolutionary ancient enzymes that have been proposed to play a key role in the calcification process, with the underlying mechanisms being little understood. Here, we used the calcifying primary mesenchyme cells (PMCs) of sea urchin larva to study the role of cytosolic (iCAs) and extracellular carbonic anhydrases (eCAs) in the cellular carbon concentration mechanism (CCM). Molecular analyses identified iCAs and eCAs in PMCs and highlight the prominent expression of a glycosylphosphatidylinositol-anchored membrane-bound CA (Cara7). Intracellular pH recordings in combination with CO2 pulse experiments demonstrated iCA activity in PMCs. iCA activity measurements, together with pharmacological approaches, revealed an opposing contribution of iCAs and eCAs on the CCM. H+-selective electrodes were used to demonstrate eCA-catalyzed CO2 hydration rates at the cell surface. Knockdown of Cara7 reduced extracellular CO2 hydration rates accompanied by impaired formation of specific skeletal segments. Finally, reduced pHi regulatory capacities during inhibition and knockdown of Cara7 underscore a role of this eCA in cellular HCO3- uptake. This work reveals the function of CAs in the cellular CCM of a marine calcifying animal. Extracellular hydration of metabolic CO2 by Cara7 coupled to HCO3- uptake mechanisms mitigates the loss of carbon and reduces the cellular proton load during the mineralization process. The findings of this work provide insights into the cellular mechanisms of an ancient biological process that is capable of utilizing CO2 to generate a versatile construction material.
Arshinoff,
Echinobase: leveraging an extant model organism database to build a knowledgebase supporting research on the genomics and biology of echinoderms.
2022, Pubmed,
Echinobase
Arshinoff,
Echinobase: leveraging an extant model organism database to build a knowledgebase supporting research on the genomics and biology of echinoderms.
2022,
Pubmed
,
Echinobase
Becker,
Carbonic anhydrase IX and acid transport in cancer.
2020,
Pubmed
Beniash,
Cellular control over spicule formation in sea urchin embryos: A structural approach.
1999,
Pubmed
,
Echinobase
Bentov,
The role of seawater endocytosis in the biomineralization process in calcareous foraminifera.
2009,
Pubmed
Bertucci,
Carbonic anhydrases in anthozoan corals-A review.
2013,
Pubmed
Bonanno,
Effect of acetazolamide on intracellular pH and bicarbonate transport in bovine corneal endothelium.
1995,
Pubmed
Brownlee,
Coccolithophore biomineralization: New questions, new answers.
2015,
Pubmed
Chang,
An otopetrin family proton channel promotes cellular acid efflux critical for biomineralization in a marine calcifier.
2021,
Pubmed
,
Echinobase
Chow,
Carbonic anhydrase activity in developing sea urchin embryos.
1979,
Pubmed
,
Echinobase
Decker,
Skeletogenesis in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Eisenhaber,
Prediction of potential GPI-modification sites in proprotein sequences.
1999,
Pubmed
Furla,
Sources and mechanisms of inorganic carbon transport for coral calcification and photosynthesis.
2000,
Pubmed
Hu,
Cellular bicarbonate accumulation and vesicular proton transport promote calcification in the sea urchin larva.
2020,
Pubmed
,
Echinobase
Hu,
A SLC4 family bicarbonate transporter is critical for intracellular pH regulation and biomineralization in sea urchin embryos.
2018,
Pubmed
,
Echinobase
Jackson,
Sponge paleogenomics reveals an ancient role for carbonic anhydrase in skeletogenesis.
2007,
Pubmed
Kahil,
Cellular pathways of calcium transport and concentration toward mineral formation in sea urchin larvae.
2020,
Pubmed
,
Echinobase
Karakostis,
Characterization of an Alpha Type Carbonic Anhydrase from Paracentrotus lividus Sea Urchin Embryos.
2016,
Pubmed
,
Echinobase
Lindskog,
Structure and mechanism of carbonic anhydrase.
1997,
Pubmed
Livingston,
A genome-wide analysis of biomineralization-related proteins in the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Love,
Gene expression patterns in a novel animal appendage: the sea urchin pluteus arm.
2007,
Pubmed
,
Echinobase
Lucas,
A physiological evaluation of carbon sources for calcification in the octocoral Leptogorgia virgulata (Lamarck).
1997,
Pubmed
Mann,
Proteomic analysis of sea urchin (Strongylocentrotus purpuratus) spicule matrix.
2010,
Pubmed
,
Echinobase
Mitsunaga,
Carbonic anhydrase activity in developing sea urchin embryos with special reference to calcification of spicules.
1986,
Pubmed
,
Echinobase
Morgulis,
Possible cooption of a VEGF-driven tubulogenesis program for biomineralization in echinoderms.
2019,
Pubmed
,
Echinobase
Moya,
Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization.
2008,
Pubmed
Newman,
A physiological measure of carbonic anhydrase in Müller cells.
1994,
Pubmed
Nimer,
Extracellular carbonic anhydrase facilitates carbon dioxide availability for photosynthesis in the marine dinoflagellate prorocentrum micans.
1999,
Pubmed
Pan,
Experimental ocean acidification alters the allocation of metabolic energy.
2015,
Pubmed
,
Echinobase
Petersen,
Na+/H+ exchangers differentially contribute to midgut fluid sodium and proton concentration in the sea urchin larva.
2021,
Pubmed
,
Echinobase
Price,
Expression of Human Carbonic Anhydrase in the Cyanobacterium Synechococcus PCC7942 Creates a High CO(2)-Requiring Phenotype : Evidence for a Central Role for Carboxysomes in the CO(2) Concentrating Mechanism.
1989,
Pubmed
Rafiq,
Genome-wide analysis of the skeletogenic gene regulatory network of sea urchins.
2014,
Pubmed
,
Echinobase
Romero,
The SLC4 family of bicarbonate (HCO₃⁻) transporters.
2013,
Pubmed
Saarikoski,
Simultaneous measurement of intracellular and extracellular carbonic anhydrase activity in intact muscle fibres.
1992,
Pubmed
Sharker,
Molecular Characterization of Carbonic Anhydrase II (CA II) and Its Potential Involvement in Regulating Shell Formation in the Pacific Abalone, Haliotis discus hannai.
2021,
Pubmed
Sly,
Human carbonic anhydrases and carbonic anhydrase deficiencies.
1995,
Pubmed
Stecher,
Molecular Evolutionary Genetics Analysis (MEGA) for macOS.
2020,
Pubmed
Sterling,
A transport metabolon. Functional interaction of carbonic anhydrase II and chloride/bicarbonate exchangers.
2001,
Pubmed
Stumpp,
Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification.
2012,
Pubmed
,
Echinobase
Stumpp,
CO2 induced seawater acidification impacts sea urchin larval development I: elevated metabolic rates decrease scope for growth and induce developmental delay.
2011,
Pubmed
,
Echinobase
Suffrian,
Cellular pH measurements in Emiliania huxleyi reveal pronounced membrane proton permeability.
2011,
Pubmed
Sun,
Signal-dependent regulation of the sea urchin skeletogenic gene regulatory network.
2014,
Pubmed
,
Echinobase
Supuran,
Carbonic anhydrases--an overview.
2008,
Pubmed
Taylor,
A voltage-gated H+ channel underlying pH homeostasis in calcifying coccolithophores.
2011,
Pubmed
Thoms,
Model of the carbon concentrating mechanism in chloroplasts of eukaryotic algae.
2001,
Pubmed
Todgham,
Transcriptomic response of sea urchin larvae Strongylocentrotus purpuratus to CO2-driven seawater acidification.
2009,
Pubmed
,
Echinobase
Trimborn,
Inorganic carbon acquisition in potentially toxic and non-toxic diatoms: the effect of pH-induced changes in seawater carbonate chemistry.
2008,
Pubmed
Tripp,
Carbonic anhydrase: new insights for an ancient enzyme.
2001,
Pubmed
Vidavsky,
Calcium transport into the cells of the sea urchin larva in relation to spicule formation.
2016,
Pubmed
,
Echinobase
Walton,
Genomics and expression profiles of the Hedgehog and Notch signaling pathways in sea urchin development.
2006,
Pubmed
,
Echinobase
Wilt,
Biomineralization of the spicules of sea urchin embryos.
2002,
Pubmed
,
Echinobase
Winter,
Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization.
2021,
Pubmed
,
Echinobase
Zhao,
A Novel Stopped-Flow Assay for Quantitating Carbonic-Anhydrase Activity and Assessing Red-Blood-Cell Hemolysis.
2017,
Pubmed