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Figure 1. Fluorescence microscopy of the cleavage site of the intestine. Images taken 3 h after autotransplantation of labeled coelomocytes to the eviscerated animal. Left panel: Differential interference contrast. Right panel: Image showing the fluorescence. Full line: Serosa. Dotted line: Cleavage site. →: cellular aggregates. Scale bars = 200 μm.
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Figure 2. Time-lapse observation of the movement of cells toward a piece of intestine. Images were acquired every 15 min. Magenta line: A piece of intestine. Arrow: Cells moved toward the growing aggregates. Arrowhead: Cells moved toward the piece of intestine. Scale bars = 200 µm.
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Figure 3. Microscopic observation of cellular aggregates with (Int+) or without (Int-) an intestinal piece on a glass slide. (A) Aggregates formed in the presence and absence of the intestine at 30 min. Scale bars = 100 μm. (B) Comparison of the total area of cell aggregates. *P < 0.05 (mean ± SD, n = 5).
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Figure 4. Aggregation-promoting activity of the tissue extracts. Total area of the aggregates, 30 min after the addition of the extracts to the coelomic fluid. P < 0.05 (mean ± SD, n = 5). Different letters show significant differences.
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Figure 5. Aggregation-promoting activity of the treated extracts of the intestine. (A) untreated extract. (B) heat-treated extract. (C, D) >5 kDa and <5 kDa fractions of the extract separated by ultrafiltration. (E) control (coelomic fluid only). Scale bars = 200 μm.
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Figure 6. Purification of the aggregation-promoting factor from the crude protein fraction of the intestinal extract. (A) Chromatogram after anion-exchange chromatography on a POROS HQ/20 column. The fraction with the activity is shaded. (B) Chromatogram after gel-filtration chromatography on a Superdex 200 column. The shaded fraction in
Figure 4A
was applied. A fraction with the activity is shaded. (C) SDS-PAGE of the shaded fraction in
Figure 4B
. A 15% polyacrylamide gel was used and was visualized by staining with coomassie brilliant blue. M, marker.
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Figure 7. Inhibitory effect of galactose on cellular aggregation. Microscopic observation. Coelomic fluid was supplemented with (A) the intestinal extract, (B) PBS, or (C–M) the intestinal extract premixed with 0.1 M sugar solution (in PBS). (C) D (+)-glucose. (D) D (+)-mannose. (E) D (+)-galactose. (F) D (+)-xylose. (G) L (−)-fucose. (H) L (+)-rhamnose. (I)
N-acetyl-D-glucosamine. (J)
N-acetyl-D-galactosamine. (K) D (+)-maltose. (L) D (+)-lactose. (M) D (+)-sucrose. Scale bars = 200 μm.
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Figure 8. Purification of the aggregation-promoting factor from the intestinal extract by galactose-affinity chromatography. (A) SDS-PAGE. Lanes: 1, molecular mass maker; 2, Intestinal extract; 3, non-adsorbed; 4, rinse-off; 5, galactose-binding protein. (B) Aggregation-promoting effects of each fraction. The numbers on the panels correspond to the number of the lanes in
Figure 7A
.Microscopic observation. Scale bars = 200 μm.
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Figure 9. Identification of the aggregation-promoting factor. (A) Peptide sequences obtained by de novo sequencing of the 16 kDa protein purified by anion-exchange and gel-filtration chromatography (
Figure 5C
). The sequences obtained by de novo sequencing of the protein purified by galactose-affinity chromatography (
Figure 7A
), as well, are underlined. (B) A sequence found in RNA-seq data that is highly identical to the peptide sequences. (C) cDNA sequence and deduced amino acid sequence of AjGBCL. The sequence obtained by RNA-seq is boxed. The sequence of partial and 3′-RACE amplified cDNA confirmed by sequencing in shaded. An EPN motif is double underlined.
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Figure 10. Reverse transcription-PCR analysis of AjGBCL expression in different tissues. β-actin was used as a loading control. M, marker; BW, body wall; TE, tentacles; PV, Polian vesicle; IN, intestine; RT, respiratory tree; CC, coelomocytes; NC, negative control (without the reverse transcription of RNA).
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Figure 11. Cellular composition of the aggregates. (A) The coelomocytes of A. japonicus classified based on MG staining. a to l indicate Type-A to Type-L, respectively. Un, unidentified. Scale bars are 5 (e and f) or 10 mm (a, b, c, d, g, h, i, j, k, and l). (B) Comparison of cellular composition of aggregates formed in the presence or absence of the intestinal extract. opened bar; absence, closed bar; intestinal extract. *P < 0.05 (mean ± SD, n = 5).
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