Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Methods Mol Biol
2022 Jan 01;2450:263-291. doi: 10.1007/978-1-0716-2172-1_14.
Show Gene links
Show Anatomy links
Studying Echinodermata Arm Explant Regeneration Using Echinaster sepositus.
Ferrario C
,
Ben Khadra Y
,
Sugni M
,
Candia Carnevali MD
,
Martinez P
,
Bonasoro F
.
???displayArticle.abstract???
Echinoderms are marine invertebrate deuterostomes known for their amazing regenerative abilities throughout all life stages. Though some species can undergo whole-body regeneration (WBR), others exhibit more restricted regenerative capabilities. Asteroidea (starfish) comprise one of the few echinoderm taxa capable of undergoing WBR. Indeed, some starfish species can restore all tissues and organs not only during larval stages, but also from arm fragments as adults. Arm explants have been used to study cells, tissues and genes involved in starfish regeneration. Here, we describe methods for obtaining and studying regeneration of arm explants in starfish, in particular animal collection and husbandry, preparation of arm explants, regeneration tests, microscopic anatomy techniques (including transmission electron microscopy, TEM) used to analyze the regenerating explant tissues and cells plus a downstream RNA extraction protocol needed for subsequent molecular investigations.
Ben Khadra,
An integrated view of asteroid regeneration: tissues, cells and molecules.
2017, Pubmed,
Echinobase
Ben Khadra,
An integrated view of asteroid regeneration: tissues, cells and molecules.
2017,
Pubmed
,
Echinobase
Ben Khadra,
Regeneration in Stellate Echinoderms: Crinoidea, Asteroidea and Ophiuroidea.
2018,
Pubmed
Ben Khadra,
Wound repair during arm regeneration in the red starfish Echinaster sepositus.
2015,
Pubmed
,
Echinobase
Ben Khadra,
Re-growth, morphogenesis, and differentiation during starfish arm regeneration.
2015,
Pubmed
,
Echinobase
Ben Khadra,
Homeobox genes expressed during echinoderm arm regeneration.
2014,
Pubmed
,
Echinobase
Candia Carnevali,
Cellular and molecular mechanisms of arm regeneration in crinoid echinoderms: the potential of arm explants.
1998,
Pubmed
,
Echinobase
Candia Carnevali,
Microscopic overview of crinoid regeneration.
2001,
Pubmed
,
Echinobase
Czarkwiani,
Skeletal regeneration in the brittle star Amphiura filiformis.
2016,
Pubmed
,
Echinobase
Ferrario,
Fundamental aspects of arm repair phase in two echinoderm models.
2018,
Pubmed
,
Echinobase
Gabre,
The coelomic epithelium transcriptome from a clonal sea star, Coscinasterias muricata.
2015,
Pubmed
,
Echinobase
Gildor,
Developmental transcriptomes of the sea star, Patiria miniata, illuminate how gene expression changes with evolutionary distance.
2019,
Pubmed
,
Echinobase
Hinman,
Developmental gene regulatory network architecture across 500 million years of echinoderm evolution.
2003,
Pubmed
,
Echinobase
Thorndyke,
Molecular approach to echinoderm regeneration.
2001,
Pubmed
,
Echinobase
Wilkie,
Mutable collagenous tissue: overview and biotechnological perspective.
2005,
Pubmed
,
Echinobase
