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Molecules
2022 Mar 27;277:. doi: 10.3390/molecules27072169.
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A Potential New Source of Therapeutic Agents for the Treatment of Mucocutaneous Leishmaniasis: The Essential Oil of Rhaphiodon echinus.
Oliveira CVB
,
Silva PAGD
,
Tintino SR
,
Coronel CC
,
Gomez MCV
,
Rolón M
,
Cunha FABD
,
Morais-Braga MFB
,
Coutinho HDM
,
Siyadatpanah A
,
Wilairatana P
,
Kamdem JP
,
Barros LM
,
Duarte AE
,
Pereira PS
.
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Weeds are an important source of natural products; with promising biological activity. This study investigated the anti-kinetoplastida potential (in vitro) to evaluate the cytotoxicity (in vitro) and antioxidant capacity of the essential oil of Rhaphiodon echinus (EORe), which is an infesting plant species. The essential oil was analyzed by GC/MS. The antioxidant capacity was evaluated by reduction of the DPPH radical and Fe3+ ion. The clone Trypanosoma cruzi CL-B5 was used to search for anti-epimastigote activity. Antileishmanial activity was determined using promastigotes of Leishmania braziliensis (MHOM/CW/88/UA301). NCTC 929 fibroblasts were used for the cytotoxicity test. The results showed that the main constituent of the essential oil was γ-elemene. No relevant effect was observed concerning the ability to reduce the DPPH radical; only at the concentration of 480 μg/mL did the essential oil demonstrate a high reduction of Fe3+ power. The oil was active against L. brasiliensis promastigotes; but not against the epimastigote form of T. cruzi. Cytotoxicity for mammalian cells was low at the active concentration capable of killing more than 70% of promastigote forms. The results revealed that the essential oil of R. echinus showed activity against L. brasiliensis; positioning itself as a promising agent for antileishmanial therapies.
Figure 1. Co-occurrence analysis of components obtained in the essential oil of Rhaphiodon echinus. Note: the size of the circle or node is equivalent to the occurrence number of the descriptor.
Figure 2. (A) Reduction of DPPH radicals by the essential oil of Rhaphiodon echinus leaves. Data are expressed as mean ± SEM of n = four independent experiments. (B) Reduction of Fe3+ to Fe2+ (110 µM) by the essential oil from Rhaphiodon echinus leaf (1–480 µg/mL). The oil was incubated for 10, 20, 30, 50, 100, and 200 min.
Figure 3. Cytotoxicity of the essential oil of Rhaphiodon echinus. As for the absence of error bars, the software used does not efficiently present such graphic elements since they have a negligible value [16].
Figure 4. Survival of Leishmania brasiliensis promastigotes and Trypanosoma cruzi epimastigotes treated with essential oil of Rhaphiodon echinus, and their respective LC50 values.
Allahverdiyev,
Antileishmanial effect of silver nanoparticles and their enhanced antiparasitic activity under ultraviolet light.
2011, Pubmed
Allahverdiyev,
Antileishmanial effect of silver nanoparticles and their enhanced antiparasitic activity under ultraviolet light.
2011,
Pubmed
Anthony,
Plant active components - a resource for antiparasitic agents?
2005,
Pubmed
Barros,
Chemical Characterization and Trypanocidal, Leishmanicidal and Cytotoxicity Potential of Lantana camara L. (Verbenaceae) Essential Oil.
2016,
Pubmed
Benelli,
High toxicity of camphene and γ-elemene from Wedelia prostrata essential oil against larvae of Spodoptera litura (Lepidoptera: Noctuidae).
2018,
Pubmed
Cheikh-Ali,
Composition, and antimicrobial and remarkable antiprotozoal activities of the essential oil of rhizomes of Aframomum sceptrum K. Schum. (Zingiberaceae).
2011,
Pubmed
Coté,
Anti-Inflammatory, Antioxidant, Antibiotic, and Cytotoxic Activities of Tanacetum vulgare L. Essential Oil and Its Constituents.
2017,
Pubmed
Duarte,
Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs.
2016,
Pubmed
,
Echinobase
Hamedt,
Cytotoxic, mutagenic and genotoxic evaluation of crude extracts and fractions from Piper jericoense with trypanocidal action.
2014,
Pubmed
Jorda,
Anti-leishmanial activity of disubstituted purines and related pyrazolo[4,3-d]pyrimidines.
2011,
Pubmed
Kamdem,
In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss.
2012,
Pubmed
Khan,
ROS regulate differentiation of visceralizing Leishmania species into the virulent amastigote form.
2018,
Pubmed
Martínez-Díaz,
Trypanocidal, trichomonacidal and cytotoxic components of cultivated Artemisia absinthium Linnaeus (Asteraceae) essential oil.
2015,
Pubmed
Mikus,
A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue.
2000,
Pubmed
Monzote,
Chemistry, cytotoxicity and antileishmanial activity of the essential oil from Piper auritum.
2010,
Pubmed
Morais-Braga,
Phenol composition, cytotoxic and anti-kinetoplastidae activities of Lygodium venustum SW. (Lygodiaceae).
2013,
Pubmed
Moreno,
Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).
2018,
Pubmed
Moura do Carmo,
Chemical and biological analyses of the essential oils and main constituents of Piper species.
2012,
Pubmed
Mukherjee,
Roles for mitochondria in pentamidine susceptibility and resistance in Leishmania donovani.
2006,
Pubmed
NULL,
Chagas disease (American trypanosomiasis) fact sheet (revised in June 2010).
2010,
Pubmed
Neub,
Cutaneous infection with Leishmania infantum in an infant treated successfully with miltefosine.
2008,
Pubmed
Niksic,
Cytotoxicity screening of Thymus vulgaris L. essential oil in brine shrimp nauplii and cancer cell lines.
2021,
Pubmed
Pereira,
Cytotoxic and anti-kinetoplastid potential of the essential oil of Alpinia speciosa K. Schum.
2018,
Pubmed
Pereira,
Cytotoxicity of Essential Oil Cordia verbenaceae against Leishmania brasiliensis and Trypanosoma cruzi.
2021,
Pubmed
Plano,
Selenocyanates and diselenides: a new class of potent antileishmanial agents.
2011,
Pubmed
Polanco-Hernández,
Synergistic Effect of Lupenone and Caryophyllene Oxide against Trypanosoma cruzi.
2013,
Pubmed
Rim,
Acaricidal effects of herb essential oils against Dermatophagoides farinae and D. pteronyssinus (Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pulegium(pennyroyal).
2006,
Pubmed
Rolón,
Selective activity of polyene macrolides produced by genetically modified Streptomyces on Trypanosoma cruzi.
2006,
Pubmed
Rondon,
In vitro efficacy of Coriandrum sativum, Lippia sidoides and Copaifera reticulata against Leishmania chagasi.
2012,
Pubmed
Saeidnia,
Biogenic trypanocidal sesquiterpenes: lead compounds to design future trypanocidal drugs - a mini review.
2013,
Pubmed
Senthilkumar,
Chemical constituents and larvicidal property of the essential oil of Blumea mollis (D. Don) Merr. against Culex quinquefasciatus.
2008,
Pubmed
Szuster-Ciesielska,
Betulin, betulinic acid and butein are inhibitors of acetaldehyde-induced activation of liver stellate cells.
2011,
Pubmed
Tariku,
In vitro evaluation of antileishmanial activity and toxicity of essential oils of Artemisia absinthium and Echinops kebericho.
2011,
Pubmed
Vega,
A new pharmacological screening assay with Trypanosoma cruzi epimastigotes expressing beta-galactosidase.
2005,
Pubmed
Yamagata,
Control of Chagas disease.
2006,
Pubmed
Yao,
Leishmania spp. and leishmaniasis on the Caribbean islands.
2020,
Pubmed
de Oliveira,
GC-MS Chemical Characterization and In Vitro Evaluation of Antioxidant and Toxic Effects Using Drosophila melanogaster Model of the Essential Oil of Lantana montevidensis (Spreng) Briq.
2019,
Pubmed
van Eck,
Software survey: VOSviewer, a computer program for bibliometric mapping.
2010,
Pubmed
