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Sci Rep
2022 May 17;121:8209. doi: 10.1038/s41598-022-12192-7.
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Cytotoxic and antimicrobial activities of two new sesquiterpenoids from red sea brittle star Ophiocoma dentata.
El Feky SE
,
Abd El Hafez MSM
,
Abd El Moneim NA
,
Ibrahim HAH
,
Okbah MA
,
Ata A
,
El Sedfy AS
,
Hussein A
.
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Bioactive compounds were extracted from a locally available brittle star; Ophiocoma dentata, collected from the Red Sea, Egypt. Two new sesquiterpenoids; 8, 11-epoxy-9(15)-himachaladiene-4-ol (O8-ophiocomane) and, 11-epoxy-9(15)-himachaladiene-4-ol (O7-ophiocomane) were isolated and characterized using appropriate techniques. Structure elucidation was estimated via 1D NMR, 2D NMR, FT-IR and mass spectroscopy analyses. The isolated compounds were tested for cytotoxic, antibacterial and antifungal activities. Pure compounds showed a dose dependent reduction in MCF-7 cells viability with LC50 of 103.5 and 59.5 μg/ml for compounds 1 and 2 respectively compared to the chemotherapeutic drug cisplatin (47.4 µg/ml). In vivo experiments showed that O. dentate extract significantly reduced tumor progression and improved hematological parameters and liver functions of tumor-bearing mice when administered either before or after tumor cells' injection. The most remarkable antimicrobial effects of O. dentate crude extract were against Staphylococcus aureus, Vibrio damsela and Pseudomonas aeruginosa while the pure compounds showed activity against P. aeruginosa alone. Neither the crude extract nor the pure compounds have shown activity against Aeromonas hydrophila. These results indicates that O. dentata extract and newly isolated compounds have shown a promising cytotoxic, antiproliferative and antimicrobial activities that might be useful for pharmaceutical applications.
Figure 1. Flow chart pure compounds’ extraction from O. dentate.
Figure 2. The 250 fractions combined (with the same RF value) fractions.
Figure 3. The (a) GC/MS and (b) FT-IR spectra of compound 1 (O8-Ophiocomane).
Figure 4. The (a) GC/MS and (b) FT-IR spectra of compound 2 (O7-Ophiocomane).
Figure 5. Cytotoxicity of O. dentata against MCF-7 cells: the graphs shows the cytotoxicity of (a) compound 1 and (b) compound 2 purified from O. dentata compared to (c) cisplatin.
Figure 6. In vivo biological effects of O. dentata in Ehrlich tumor-bearing mice including (a) tumor size measurements (b) hematological parameters and liver function tests in study groups.
Figure 7. Histopathological analysis of liver in vivo study using laboratory animal for cytotoxicity and anticancer activity, where picture (a) liver of a healthy control mouse, (b) liver of tumor-bearing mouse treated with O. dentata extract with no abnormal changes. (c) Ehrlich tumors of untreated mouse. (d) Ehrlich tumor of O. dentata treated mouse showing deeply chromatic pleomorphic nuclei with wide areas of necrosis.
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