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Oxid Med Cell Longev
2022 Jan 01;2022:8186838. doi: 10.1155/2022/8186838.
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Preventive Effect of Collagen Peptides from Acaudina molpadioides on Acute Kidney Injury through Attenuation of Oxidative Stress and Inflammation.
Zhao W
,
Li J
,
Li Y
,
Chen Y
,
Jin H
.
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The protective effect of collagen peptide from Acaudina molpadioides (Amp) on acute kidney injury (AKI) in mice and its mechanism were explored. The results showed that Amp-fed could effectively improve the renal mass index and histopathological morphology. The levels of serum creatinine and urea nitrogen decreased significantly, while the antioxidant enzyme catalase (CAT) and superoxide dismutase (SOD) increased significantly in Amp-fed groups. Western blot results disclosed that Amp significantly upregulates the levels of heme oxygenase-1 (HO-1), Nrf2, p-PI3K, and p-AKT in the kidney. In addition, Amp could significantly downregulate the levels of nuclear factor NF-kappa-B (NF-κB), tumor necrosis factor α (TNF-α), inflammatory cytokines interleukin 6 (IL-6) and interleukin 1β (IL-1β). These findings provide evidence that Amp plays a protective role in AKI via attenuation of oxidative stress and inflammation mediated by PI3K/AKT/Nrf2 and PI3K/AKT/NF-κB pathways. This study laid a foundation for the application of Amp in the prevention of AKI.
Figure 1. Changes in the kidney pathology (a–f) and the renal mass index (g) in Amp-fed mice with CCl4 administration. Control group (a); CCl4 group (b); VE group (c); 50 mg/kg Amp group (d); 100 mg/kg Amp group (e); 200 mg/kg Amp group (f). H&E staining (400x) with eight mice in each group. Data are expressed as the mean ± SD (n = 8 in each group). ∗p < 0.05, compared to the CCl4 group.
Figure 2. Effects of Amp-fed on CRE (a) and BUN (b) levels in kidney of CCl4- induced mice. Data are expressed as the mean ± SD (n = 8 in each group). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001, compared to the CCl4 group.
Figure 3. Effects of Amp-fed on the activities of CAT (a) and SOD (b) in the kidney of CCl4-treated mice. Data are expressed as the mean ± SD (n = 8 in each group). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001, compared to the CCl4 group.
Figure 4. Effect of Amp-fed on the protein levels of the Keap1/Nrf2-ARE signaling pathway. Data are expressed as the mean ± SD (n = 8 in each group). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001, compared to the CCl4 group.
Figure 5. Effect of Amp-fed on the levels of IL-1β (a), IL-6 (b), TNF-α (c), and NF-κB (d) in CCl4-induced mice. Data are expressed as the mean ± SD (n = 8 in each group). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001, compared to the CCl4 group.
Figure 6. Effect of Amp-fed on the activation of PI3K/AKT pathway in kidney of CCl4-induced mice. Data are expressed as the mean ± SD (n = 8 in each group). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001, compared to the CCl4 group.
Figure 7. Molecular mechanism of Amp's protective effect on CCl4-induced acute kidney injury through PI3K/AKT-mediated Nrf2 and NF-κB signaling pathways.
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