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Foods
2021 Jun 04;106:. doi: 10.3390/foods10061294.
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Amino Acid Profiling with Chemometric Analysis as a Feasible Tool for the Discrimination of Marine-Derived Peptide Powders.
Wang Q
,
Wang Y
,
Jiang X
,
Ma L
,
Li Z
,
Chang Y
,
Wang Y
,
Xue C
.
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Marine-derived peptide powders have suffered from adulteration via the substitution of lower-price peptides or the addition of adulterants in the market. This study aims to establish an effective approach for the discrimination and detection of adulterants for four representative categories of marine-derived peptide powders, namely, oyster peptides, sea cucumber peptides, Antarctic krill peptides, and fish skin peptides, based on amino acid profiling alongside chemometric analysis. The principal component analysis and orthogonal partial least squares discriminant analysis results indicate that four categories of marine-derived peptides could be distinctly classified into four clusters and aggregated with the respective raw materials. Taurine, glycine, lysine, and protein contents were the major discriminants. A reliable classification model was constructed and validated by the prediction dataset, mixture sample dataset, and unclassified sample dataset with accuracy values of 100%, 100%, and 100%, respectively.
31801479 National Natural Science Foundation of China, 2018SDKJ0304-2 Marine Science and Technology Fund of Shandong Province, 862001013136 Fundamental Research Funds for the Central Universities of China
Figure 1. Chemometric analysis. (A) Score plot of the PCA for raw materials. (B) Loading plot of the PCA for raw materials. (C) Score plot of the OPLS–DA for raw materials. (D) VIP plot of the OPLS–DA for raw materials. (E) Score plot of the PCA for marine-derived peptides. (F) Loading plot of the PCA for marine-derived peptides. (G) Score plot of the OPLS–DA for marine-derived peptides. (H) VIP plot of the OPLS–DA for marine-derived peptides.
Figure 2. Chemometric analysis for the marine-derived peptides combined with the respective raw materials. (A) Score plot of the PCA model. (B) Loading plot of the PCA model. (C) Score plot of the OPLS–DA model. (D) VIP plot of the OPLS–DA model.
Figure 3. Classification model for the marine-derived peptides. (A) DModX PS+ column plot of the oyster group. (B) DModX PS+ column plot of the sea cucumber group. (C) DModX PS+ column plot of the Antarctic krill group. (D) DModX PS+ column plot of the fish skin group. (E) Misclassification table of the training set. (F) Misclassification table of the prediction set.
Figure 4. Application of the classification model based on the DModX PS+ column plot for adulteration detection using (A–D) the marine-derived peptide mixture sample dataset and (E–H) the unclassified peptide sample dataset.
Asadpoor,
Amino Acid profile as a feasible tool for determination of the authenticity of fruit juices.
2014, Pubmed
Asadpoor,
Amino Acid profile as a feasible tool for determination of the authenticity of fruit juices.
2014,
Pubmed
Botoran,
Amino Acid Profile of Fruits as Potential Fingerprints of Varietal Origin.
2019,
Pubmed
Chen,
Discrimination of botanical origins for Chinese honey according to free amino acids content by high-performance liquid chromatography with fluorescence detection with chemometric approaches.
2017,
Pubmed
Chen,
Amino acid and mineral composition of protein and other components and their recovery yields from whole Antarctic krill (Euphausia superba) using isoelectric solubilization/precipitation.
2009,
Pubmed
Fiamegos,
Authentication of PDO paprika powder (Pimentón de la Vera) by multivariate analysis of the elemental fingerprint determined by ED-XRF. A feasibility study.
2021,
Pubmed
Hajimahmoodi,
Gas-chromatographic fatty-acid fingerprints and partial least squares modeling as a basis for the simultaneous determination of edible oil mixtures.
2005,
Pubmed
Hong,
Modern analytical methods for the detection of food fraud and adulteration by food category.
2017,
Pubmed
Kotsanopoulos,
The use of molecular markers in the verification of fish and seafood authenticity and the detection of adulteration.
2021,
Pubmed
Lerma-García,
Classification of vegetable oils according to their botanical origin using amino acid profiles established by direct infusion mass spectrometry.
2007,
Pubmed
Li,
Mechanism of aroma compounds changes from sea cucumber peptide powders (SCPPs) under different storage conditions.
2020,
Pubmed
,
Echinobase
Li,
Determination of the authenticity of plastron-derived functional foods based on amino acid profiles analysed by MEKC.
2017,
Pubmed
Manikkam,
A Review of Potential Marine-derived Hypotensive and Anti-obesity Peptides.
2016,
Pubmed
Manninen,
Protein hydrolysates in sports nutrition.
2009,
Pubmed
Nicolaou,
Fourier transform infrared spectroscopy and multivariate analysis for the detection and quantification of different milk species.
2010,
Pubmed
Pavlicevic,
Marine Bioactive Peptides-An Overview of Generation, Structure and Application with a Focus on Food Sources.
2020,
Pubmed
Qian,
Antioxidant and anti-inflammatory peptide fraction from oyster soft tissue by enzymatic hydrolysis.
2020,
Pubmed
Rebane,
Evaluation of the botanical origin of estonian uni- and polyfloral honeys by amino acid content.
2008,
Pubmed
Reis Lima,
Serra da Estrela cheese's free amino acids profiles by UPLC-DAD-MS/MS and their application for cheese origin assessment.
2019,
Pubmed
Song,
In situ and real-time authentication of Thunnus species by iKnife rapid evaporative ionization mass spectrometry based lipidomics without sample pretreatment.
2020,
Pubmed
Tezcan,
Determination of amino acids in pomegranate juices and fingerprint for adulteration with apple juices.
2013,
Pubmed
Wen,
Chemical composition and nutritional quality of sea cucumbers.
2010,
Pubmed
,
Echinobase
Wistaff,
Chemometric analysis of amino acid profiles for detection of fruit juice adulterations - Application to verify authenticity of blood orange juice.
2021,
Pubmed
Ye,
Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicus in an endurance swimming rat model.
2017,
Pubmed
,
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