Cell Calcium 2021 Nov 01;99:102466. doi: 10.1016/j.ceca.2021.102466.

CatSper channels in sea urchin sperm.

???displayArticle.abstract???
Sea urchin sperm swimming is regulated by speract, a decapeptide released from egg jelly that induces chemotaxis and triggers membrane potential (Em) changes, intracellular increases in cyclic nucleotides (cGMP, cAMP), pH (pHi) and calcium concentration ([Ca2+]i). The identity of the ionic transporters associated with the [Ca2+]i changes required for chemotaxis is not fully known. CatSper, a sperm exclusive Ca2+ channel has been detected by proteomic analysis and immunofluorescence in sea urchin sperm and there is evidence for its involvement in chemotaxis. This work presents an electrophysiological characterization of a CatSper channel in sea urchin sperm. By swelling sperm suspending them in 10-fold diluted artificial sea water (ASW) we achieve on-cell patch-clamp recordings that document a mildly voltage and pHi dependent Na+ permeable channel (in absence of divalent ions in the pipette), sensitive to speract, and blocked by Mibefradil (Mibe), NNC55-0396 (NNC) and RU1968 (RU) resembling CatSper. We also recorded a voltage dependent Cl- channel inhibited by Niflumic Acid and the TMEM16A blocker.

???displayArticle.pubmedLink??? 34509139
???displayArticle.link??? Cell Calcium


???displayArticle.antibodies??? catsper1 Ab1