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Conserv Physiol
2019 Jan 01;71:coz062. doi: 10.1093/conphys/coz062.
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The impact of environmental acidification on the microstructure and mechanical integrity of marine invertebrate skeletons.
Byrne M
,
Fitzer S
.
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Ocean acidification (OA), from seawater uptake of anthropogenic CO2, has a suite of negative effects on the ability of marine invertebrates to produce and maintain their skeletons. Increased organism pCO2 causes hypercapnia, an energetically costly physiological stress. OA alters seawater carbonate chemistry, limiting the carbonate available to form the calcium carbonate (CaCO3) minerals used to build skeletons. The reduced saturation state of CaCO3 also causes corrosion of CaCO3 structures. Global change is also accelerating coastal acidification driven by land-run off (e.g. acid soil leachates, tannic acid). Building and maintaining marine biomaterials in the face of changing climate will depend on the balance between calcification and dissolution. Overall, in response to environmental acidification, many calcifiers produce less biomineral and so have smaller body size. Studies of skeleton development in echinoderms and molluscs across life stages show the stunting effect of OA. For corals, linear extension may be maintained, but at the expense of less dense biomineral. Conventional metrics used to quantify growth and calcification need to be augmented by characterisation of the changes to biomineral structure and mechanical integrity caused by environmental acidification. Scanning electron microscopy and microcomputed tomography of corals, tube worms and sea urchins exposed to experimental (laboratory) and natural (vents, coastal run off) acidification show a less dense biomineral with greater porosity and a larger void space. For bivalves, CaCO3 crystal deposition is more chaotic in response to both ocean and coastal acidification. Biomechanics tests reveal that these changes result in weaker, more fragile skeletons, compromising their vital protective roles. Vulnerabilities differ among taxa and depend on acidification level. Climate warming has the potential to ameliorate some of the negative effects of acidification but may also make matters worse. The integrative morphology-ecomechanics approach is key to understanding how marine biominerals will perform in the face of changing climate.
Figure 1. Equations for the mechanisms of acidification from CO2-OA and other forms of environmental acidification for example from acid sulphate soil leachates. Modified from Fitzer et al. (2018).
Figure 2. A schematic representation of the use of SEM-EBSD as an analytical tool to assess the effects of acidification on shell microstructure. (A) M. edulis has been a focal study species to understand the impacts of OA on marine biomineral. (B) Section imaged using SEM is a cross section through a shell grown under OA (pHNBS 7.5). (C) The same section imaged using EBSD and analysed for crystallographic orientation displayed as a crystallographic orientation map. (D) Calcite and (E) aragonite crystals at higher magnification from the same cross section of the M. edulis shell. (F) Disordered microstructure of the calcite layer and (G) dissolution of the aragonite tablets (edges more rounded compared to panel E and tablets are less tightly packed) of the shells grown in OA. Images adapted from Fitzer et al. (2014a).
Figure 3. Crystallographic orientation maps with accompanying pole figures for the calcite and aragonite shell of M. edulis (A, D), and the calcite shells of Magallana angulata (B, E) and Saccostrea glomerata (C, F) grown at pH 8.1NBS and pHNBS 7.5 under CO2 acidfication and sulphate soil acidification. The figures highlight the similarly altered crystallographic orientation of the mussel and oyster shells with increased disorder at pH 7.5. This is highlighted by the increased range of crystallographic orientation shown by the increased variation of colours. The colours here represent a change in the angle of crystallographic orientation as per the calcite (0001) (G) and aragonite (001) (H) colour keys. Scale bars represent 5 μm for M. edulis, 45 μm for M. angulata and 200 μm for S. glomerata. Adapted from Fitzer et al. (2014a, 2018) and Meng et al. (2018).
Figure 4. SEM of the surface of the apical test plates of the adult sea urchin, Heliocidaris erythrogramma maintained in control (pHNBS 8.1) (A) and decreased pH (pHNBS 7.6) (B) for 9 months. The skeleton formed in the OA treatment has thinner calcite. Images courtesy of Ms R. Johnson.
Figure 5. SEM of juvenile Heliocidaris erythrogramma reared in four pH and three temperature levels in all combinations for 14 days. Urchins had shorter spines and smaller tests at pHNBS 7.4 (see Wolfe et al. 2013b). At control pH (A, E) the arrows point to the terminal spike which is the calcite growing region of the spines compared with the flat-ended spines of juveniles reared in pHNBS 7.4 indicating retarded or no calcification. Images courtesy of Dr K Wolfe.
Figure 6. SEM of the spines of juvenile Heliocidaris erythrogramma reared in four pH and three temperature levels in all combinations for 14 days. The arrows point to the pointed end of the spines in control pHNBS 8.1 (A, E) at the calcite growing region. At pHNBS 7.4 and at warmer temperature the spines were shorter, more porous, had blunt ends (D) and were eroded (H) (see Wolfe et al. 2013b). Images courtesy of Dr K Wolfe.
Figure 7.
Tripneustes gratilla reared in three pH and three temperature levels in all combinations from the early juvenile (5.0 mm test diameter) for 146 days. A +3°C warming mitigated the negative effects of low pHNBS (pH 7.6, 7.8), but further warming was deleterious. From Dworjanyn and Byrne (2018).
Albright,
Juvenile growth of the tropical sea urchin Lytechinus variegatus exposed to near-future ocean acidification scenarios.
2012, Pubmed,
Echinobase
Albright,
Juvenile growth of the tropical sea urchin Lytechinus variegatus exposed to near-future ocean acidification scenarios.
2012,
Pubmed
,
Echinobase
Andersson,
Ocean acidification and coral reefs: effects on breakdown, dissolution, and net ecosystem calcification.
2013,
Pubmed
Asnaghi,
Cascading effects of ocean acidification in a rocky subtidal community.
2013,
Pubmed
,
Echinobase
Asnaghi,
Bottom-up effects on biomechanical properties of the skeletal plates of the sea urchin Paracentrotus lividus (Lamarck, 1816) in an acidified ocean scenario.
2019,
Pubmed
,
Echinobase
Asnaghi,
Effects of ocean acidification and diet on thickness and carbonate elemental composition of the test of juvenile sea urchins.
2014,
Pubmed
,
Echinobase
Bibby,
Ocean acidification disrupts induced defences in the intertidal gastropod Littorina littorea.
2007,
Pubmed
Byrne,
Warming influences Mg2+ content, while warming and acidification influence calcification and test strength of a sea urchin.
2014,
Pubmed
,
Echinobase
Byrne,
The stunting effect of a high CO2 ocean on calcification and development in sea urchin larvae, a synthesis from the tropics to the poles.
2013,
Pubmed
,
Echinobase
Calosi,
Distribution of sea urchins living near shallow water CO2 vents is dependent upon species acid-base and ion-regulatory abilities.
2013,
Pubmed
,
Echinobase
Carey,
Sea urchins in a high-CO2 world: partitioned effects of body size, ocean warming and acidification on metabolic rate.
2016,
Pubmed
,
Echinobase
Chan,
CO(2)-driven ocean acidification alters and weakens integrity of the calcareous tubes produced by the serpulid tubeworm, Hydroides elegans.
2012,
Pubmed
Comeau,
Coral calcifying fluid pH is modulated by seawater carbonate chemistry not solely seawater pH.
2017,
Pubmed
Coronado,
Impact of ocean acidification on crystallographic vital effect of the coral skeleton.
2019,
Pubmed
Crook,
Reduced calcification and lack of acclimatization by coral colonies growing in areas of persistent natural acidification.
2013,
Pubmed
Dell'Acqua,
Robustness of Adamussium colbecki shell to ocean acidification in a short-term exposure.
2019,
Pubmed
Dery,
Ocean Acidification Reduces Spine Mechanical Strength in Euechinoid but Not in Cidaroid Sea Urchins.
2017,
Pubmed
,
Echinobase
Dery,
Cidaroids spines facing ocean acidification.
2018,
Pubmed
,
Echinobase
Dickinson,
Interactive effects of salinity and elevated CO2 levels on juvenile eastern oysters, Crassostrea virginica.
2012,
Pubmed
Doney,
Ocean acidification: the other CO2 problem.
2009,
Pubmed
,
Echinobase
Doubleday,
A triple trophic boost: How carbon emissions indirectly change a marine food chain.
2019,
Pubmed
Dubois,
The skeleton of postmetamorphic echinoderms in a changing world.
2014,
Pubmed
,
Echinobase
Dworjanyn,
Impacts of ocean acidification on sea urchin growth across the juvenile to mature adult life-stage transition is mitigated by warming.
2018,
Pubmed
,
Echinobase
Díaz-Castañeda,
Ocean acidification affects calcareous tube growth in adults and reared offspring of serpulid polychaetes.
2019,
Pubmed
Ellers,
Structural Strengthening of Urchin Skeletons by Collagenous Sutural Ligaments.
1998,
Pubmed
,
Echinobase
Emerson,
Ocean acidification impacts spine integrity but not regenerative capacity of spines and tube feet in adult sea urchins.
2017,
Pubmed
,
Echinobase
Fantazzini,
Gains and losses of coral skeletal porosity changes with ocean acidification acclimation.
2015,
Pubmed
Fitzer,
Ocean acidification and temperature increase impact mussel shell shape and thickness: problematic for protection?
2015,
Pubmed
Fitzer,
Biomineral shell formation under ocean acidification: a shift from order to chaos.
2016,
Pubmed
Fitzer,
Ocean acidification alters the material properties of Mytilus edulis shells.
2015,
Pubmed
Fitzer,
Ocean acidification reduces the crystallographic control in juvenile mussel shells.
2014,
Pubmed
Fitzer,
Coastal acidification impacts on shell mineral structure of bivalve mollusks.
2018,
Pubmed
Fitzer,
Selectively bred oysters can alter their biomineralization pathways, promoting resilience to environmental acidification.
2019,
Pubmed
Fitzer,
Ocean acidification impacts mussel control on biomineralisation.
2014,
Pubmed
Gattuso,
OCEANOGRAPHY. Contrasting futures for ocean and society from different anthropogenic CO₂ emissions scenarios.
2015,
Pubmed
Gaylord,
Functional impacts of ocean acidification in an ecologically critical foundation species.
2011,
Pubmed
González-Delgado,
The Importance of Natural Acidified Systems in the Study of Ocean Acidification: What Have We Learned?
2018,
Pubmed
Jiang,
Composition of dissolved organic matter (DOM) from periodically submerged soils in the Three Gorges Reservoir areas as determined by elemental and optical analysis, infrared spectroscopy, pyrolysis-GC-MS and thermally assisted hydrolysis and methylation.
2017,
Pubmed
Kroeker,
Predicting the effects of ocean acidification on predator-prey interactions: a conceptual framework based on coastal molluscs.
2014,
Pubmed
Kroeker,
Impacts of ocean acidification on marine organisms: quantifying sensitivities and interaction with warming.
2013,
Pubmed
Leung,
Impacts of Near-Future Ocean Acidification and Warming on the Shell Mechanical and Geochemical Properties of Gastropods from Intertidal to Subtidal Zones.
2017,
Pubmed
Li,
Weakening mechanisms of the serpulid tube in a high-CO2 world.
2014,
Pubmed
Li,
Interactive effects of seawater acidification and elevated temperature on biomineralization and amino acid metabolism in the mussel Mytilus edulis.
2015,
Pubmed
Li,
Mechanical robustness of the calcareous tubeworm Hydroides elegans: warming mitigates the adverse effects of ocean acidification.
2016,
Pubmed
Mackenzie,
Ocean warming, more than acidification, reduces shell strength in a commercial shellfish species during food limitation.
2014,
Pubmed
Marin,
Molluscan shell proteins: primary structure, origin, and evolution.
2008,
Pubmed
Melzner,
Food supply and seawater pCO2 impact calcification and internal shell dissolution in the blue mussel Mytilus edulis.
2011,
Pubmed
Meng,
Calcium carbonate unit realignment under acidification: A potential compensatory mechanism in an edible estuarine oyster.
2019,
Pubmed
Migliaccio,
Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO2 vent system.
2019,
Pubmed
,
Echinobase
Milano,
Impact of high pCO2 on shell structure of the bivalve Cerastoderma edule.
2016,
Pubmed
Mooney,
Statolith Morphometrics Can Discriminate among Taxa of Cubozoan Jellyfishes.
2016,
Pubmed
Pan,
Experimental ocean acidification alters the allocation of metabolic energy.
2015,
Pubmed
,
Echinobase
Pansch,
Habitat traits and food availability determine the response of marine invertebrates to ocean acidification.
2014,
Pubmed
Parker,
Predicting the response of molluscs to the impact of ocean acidification.
2013,
Pubmed
Przeslawski,
A review and meta-analysis of the effects of multiple abiotic stressors on marine embryos and larvae.
2015,
Pubmed
Queirós,
Scaling up experimental ocean acidification and warming research: from individuals to the ecosystem.
2015,
Pubmed
Roleda,
BEFORE OCEAN ACIDIFICATION: CALCIFIER CHEMISTRY LESSONS(1).
2012,
Pubmed
Sadler,
The effects of elevated CO2 on shell properties and susceptibility to predation in mussels Mytilus edulis.
2018,
Pubmed
Sanford,
Ocean acidification increases the vulnerability of native oysters to predation by invasive snails.
2014,
Pubmed
Stemmer,
Elevated CO₂ levels do not affect the shell structure of the bivalve Arctica islandica from the Western Baltic.
2013,
Pubmed
Stumpp,
Resource allocation and extracellular acid-base status in the sea urchin Strongylocentrotus droebachiensis in response to CO₂ induced seawater acidification.
2012,
Pubmed
,
Echinobase
Sötje,
New Methods of Morphometric Analyses on Scyphozoan Jellyfish Statoliths Including the first Direct Evidence for Statolith Growth Using Calcein as a Fluorescent Marker.
2017,
Pubmed
Tambutté,
Morphological plasticity of the coral skeleton under CO2-driven seawater acidification.
2015,
Pubmed
Uthicke,
Echinometra sea urchins acclimatized to elevated pCO2 at volcanic vents outperform those under present-day pCO2 conditions.
2016,
Pubmed
,
Echinobase
Viotti,
Effects of long-term exposure to reduced pH conditions on the shell and survival of an intertidal gastropod.
2019,
Pubmed
Von Euw,
Biological control of aragonite formation in stony corals.
2017,
Pubmed
Wolfe,
Vulnerability of the paper Nautilus (Argonauta nodosa) shell to a climate-change ocean: potential for extinction by dissolution.
2012,
Pubmed
Wolfe,
Effects of ocean warming and acidification on survival, growth and skeletal development in the early benthic juvenile sea urchin (Heliocidaris erythrogramma).
2013,
Pubmed
,
Echinobase
