Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Echinobase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Echinobase
ECB-ART-48350
Cold Spring Harb Protoc 2010 Dec 01;201012:pdb.prot5536. doi: 10.1101/pdb.prot5536.
Show Gene links Show Anatomy links

Microinjection of Xenopus oocytes.

Sive HL , Grainger RM , Harland RM .


???displayArticle.abstract???
Xenopus oocytes can be injected with messenger RNA (mRNA) or DNA constructs into the cytoplasm or nucleus, respectively. The cytoplasm can withstand the introduction of up to 50 nL of injected material, and the nucleus can tolerate up to 20 nL. This protocol describes microinjection of both defolliculated and folliculated Xenopus oocytes. Oocytes remain in good condition for a relatively long time, and injections can be continued, if necessary, over a period of days.

???displayArticle.pubmedLink??? 21123423
???displayArticle.link??? Cold Spring Harb Protoc