Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mar Drugs
2019 Jul 05;177:. doi: 10.3390/md17070397.
Show Gene links
Show Anatomy links
Quantitative Proteome Reveals Variation in the Condition Factor of Sea Urchin Strongylocentrotus nudus during the Fishing Season Using an iTRAQ-based Approach.
Shang WH
,
Han JR
,
Yan JN
,
Du YN
,
Xu YS
,
Xue CF
,
Zhang TT
,
Wu HT
,
Zhu BW
.
???displayArticle.abstract???
To investigate the variation in the condition factor of the sea urchin Strongylocentrotus nudus (S. nudus), gonads were collected in May (MAY), June (JUN), and July (JUL), at the beginning (AUG-b) and end of August (AUG-e). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) detection of the gonads revealed an obvious enhancement of the band at about 37 kDa from July, which was identified as transforming growth factor-beta-induced protein ig-h3 (TGFBI) by nanoLC-ESI-MS/MS. Gonadal proteins were identified by isobaric tagging for relative and absolute quantitation (iTRAQ), and regulation of the identified proteins in pairs of the collected groups was observed. A total of 174 differentially expressed proteins (DEPs) were identified. Seven of the DEPs showed significant correlations with both the gonad index (GI) and protein content. These correlations included 6-phosphogluconate dehydrogenase, decarboxylating isoform X2 (6PGD), CAD protein, myoferlin isoform X8, ribosomal protein L36 (RL36), isocitrate dehydrogenase [NADP], mitochondrialisoform X2 (IDH), multifunctional protein ADE2 isoform X3, sperm-activating peptides (SAPs) and aldehyde dehydrogenase, and mitochondrial (ALDH). However, TGFBI had no correlation with gonad index (GI) or protein content. 6PGD, IDH, multifunctional protein ADE2isoform X3, and ALDH were shown to interact with each other and might play key roles in changing the condition factor of S. nudus gonads.
Figure 1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of gonad proteins in S. nudus. Lane 1, protein marker. The gonads collected in May (MAY), June (JUN), July (JUL), at the beginning of August. and at the end of August are shown in lanes 2–4, 5–7, 8–10, 11–13 and 14–16, respectively. Aug-b, the beginning of August; Aug-e, the end of August.
Figure 2. The number of upregulated and downregulated differentially expressed proteins (DEPs) identified in the compared groups (JUN–MAY, JUL–JUN, AUG-b-JUL, and AUG-e-AUG-b).
Figure 3. Gene ontology (GO) annotation of the upregulated and downregulated differentially expressed proteins (DEPs) identified in compared groups (JUN–MAY, JUL–JUN, AUG-b-JUL, and AUG-e-AUG-b) including biological processes, molecular functions, and cellular components.
Figure 4. The protein-protein interaction (PPI) analysis among the differentially expressed proteins (DEPs) related to both gonad index (GI) and protein content in the compared groups (JUN–MAY, JUL–JUN, AUG-b-JUL, and AUG-e-AUG-b). Red nodes represent upregulated proteins; blue nodes represent downregulated proteins; black line: co-expression.
Figure 5. Experimental design and workflow for the quantitative proteomic analysis of S. nudus gonads using the isobaric tagging for relative and absolute quantitation (iTRAQ) labelling proteomic strategy.
Adams,
Proteomic responses of sea urchin embryos to stressful ultraviolet radiation.
2012, Pubmed,
Echinobase
Adams,
Proteomic responses of sea urchin embryos to stressful ultraviolet radiation.
2012,
Pubmed
,
Echinobase
Arafa,
The influence of season on the gonad index and biochemical composition of the sea urchin Paracentrotus lividus from the Golf of Tunis.
2012,
Pubmed
,
Echinobase
Archana,
Nutrient composition and antioxidant activity of gonads of sea urchin Stomopneustes variolaris.
2016,
Pubmed
,
Echinobase
Arcos-Hernández,
FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters.
2016,
Pubmed
,
Echinobase
Auslander,
The effect of tert-butyl hydroperoxide on hepatic transcriptome expression patterns in the striped sea bream (Lithognathus mormyrus; Teleostei).
2010,
Pubmed
Cao,
Effects of magnetic nanoparticles plus microwave on the thawing of largemouth bass (Micropterus salmoides) fillets based on iTRAQ quantitative proteomics.
2019,
Pubmed
Coxam,
carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish.
2015,
Pubmed
Czyzewska,
Changes of activity and kinetics of certain liver and heart enzymes of hypothyroid and T(3)-treated rats.
2012,
Pubmed
Du,
Validation of housekeeping genes as internal controls for studying gene expression during Pacific oyster (Crassostrea gigas) development by quantitative real-time PCR.
2013,
Pubmed
Faut,
Metabolism of ethanol to acetaldehyde and increased susceptibility to oxidative stress could play a role in the ovarian tissue cell injury promoted by alcohol drinking.
2009,
Pubmed
Garmendia,
Seasonal variability in gonad development in the sea urchin (Paracentrotus lividus) on the Basque coast (southeastern Bay of Biscay).
2010,
Pubmed
,
Echinobase
Jagadeeshan,
Evolution of gamete attraction molecules: evidence for purifying selection in speract and its receptor, in the pantropical sea urchin Diadema.
2015,
Pubmed
,
Echinobase
Jo,
Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase.
2001,
Pubmed
Johnson,
Deletion of murine choline dehydrogenase results in diminished sperm motility.
2010,
Pubmed
Jungsukcharoen,
Proteome analysis of Pueraria mirifica tubers collected in different seasons.
2016,
Pubmed
Karp,
Addressing accuracy and precision issues in iTRAQ quantitation.
2010,
Pubmed
Kawasoe,
Acquired resistance of leukemic cells to AraC is associated with the upregulation of aldehyde dehydrogenase 1 family member A2.
2013,
Pubmed
Kil,
Regulation of mitochondrial NADP+-dependent isocitrate dehydrogenase activity by glutathionylation.
2005,
Pubmed
Kohn,
Proteomic analysis of early-stage embryos: implications for egg quality in hapuku (Polyprion oxygeneios).
2015,
Pubmed
Li,
Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris.
2012,
Pubmed
Li,
iTRAQ analysis of liver immune-related proteins from darkbarbel catfish (Pelteobagrus vachelli) infected with Edwardsiella ictaluri.
2019,
Pubmed
McClatchie,
Betaine is accumulated via transient choline dehydrogenase activation during mouse oocyte meiotic maturation.
2017,
Pubmed
Oulhen,
Dysferlin is essential for endocytosis in the sea star oocyte.
2014,
Pubmed
,
Echinobase
Pastor,
Identification of proteomic signatures associated with lung cancer and COPD.
2013,
Pubmed
Savitski,
A Scalable Approach for Protein False Discovery Rate Estimation in Large Proteomic Data Sets.
2015,
Pubmed
Shang,
In silico assessment and structural characterization of antioxidant peptides from major yolk protein of sea urchin Strongylocentrotus nudus.
2018,
Pubmed
,
Echinobase
Shi,
Differential proteomic analysis to identify proteins associated with quality traits of frozen mud shrimp (Solenocera melantho) using an iTRAQ-based strategy.
2018,
Pubmed
Siliani,
Influence of seasonal and environmental patterns on the lipid content and fatty acid profiles in gonads of the edible sea urchin Paracentrotus lividus from Sardinia.
2016,
Pubmed
,
Echinobase
Swezey,
The in vivo rate of glucose-6-phosphate dehydrogenase activity in sea urchin eggs determined with a photolabile caged substrate.
1995,
Pubmed
,
Echinobase
Sánchez-Romero,
Organization of ribonucleoside diphosphate reductase during multifork chromosome replication in Escherichia coli.
2011,
Pubmed
Unuma,
Accumulation of the major yolk protein and zinc in the agametogenic sea urchin gonad.
2011,
Pubmed
,
Echinobase
Wan,
Roles of Ebp2 and ribosomal protein L36 in ribosome biogenesis in Saccharomyces cerevisiae.
2015,
Pubmed
Wang,
iTRAQ-based quantitative proteomics reveals the biochemical mechanism of cold stress adaption of razor clam during controlled freezing-point storage.
2018,
Pubmed
Wen,
IQuant: an automated pipeline for quantitative proteomics based upon isobaric tags.
2014,
Pubmed
Wu,
Proteolysis of noncollagenous proteins in sea cucumber, Stichopus japonicus, body wall: characterisation and the effects of cysteine protease inhibitors.
2013,
Pubmed
,
Echinobase
Xing,
Proteome Analysis Using Isobaric Tags for Relative and Absolute Analysis Quantitation (iTRAQ) Reveals Alterations in Stress-Induced Dysfunctional Chicken Muscle.
2017,
Pubmed
Yang,
iTRAQ protein profile analysis of Citrus sinensis roots in response to long-term boron-deficiency.
2013,
Pubmed
Zhou,
Characterization of lipids in three species of sea urchin.
2018,
Pubmed
,
Echinobase
Zhou,
Optimisation of hydrolysis of purple sea urchin (Strongylocentrotus nudus) gonad by response surface methodology and evaluation of in vitro antioxidant activity of the hydrolysate.
2012,
Pubmed
,
Echinobase
