ECB-ART-47112
Methods Cell Biol
2019 Jan 01;151:89-113. doi: 10.1016/bs.mcb.2018.10.003.
Show Gene links
Show Anatomy links
Methods for the experimental and computational analysis of gene regulatory networks in sea urchins.
???displayArticle.abstract???
The discovery of gene regulatory networks (GRNs) has opened a gate to access the genomic mechanisms controlling development. GRNs are systems of transcriptional regulatory circuits that control the differential specification of cell fates during development by regulating gene expression. The experimental analysis of GRNs involves a collection of methods, each revealing aspects of the overall control process. This review provides an overview of experimental and computational methods that have been successfully applied for solving developmental GRNs in the sea urchin embryo. The key in this approach is to obtain experimental evidence for functional interactions between transcription factors and regulatory DNA. In the second part of this review, a more generally applicable strategy is discussed that shows a path from experimental evidence to annotation of regulatory linkages to the generation of GRN models.
???displayArticle.pubmedLink??? 30948033
???displayArticle.link??? Methods Cell Biol
Genes referenced: LOC100887844 LOC115919910 LOC575170