ECB-ART-47108
Methods Cell Biol
2019 Jan 01;151:487-517. doi: 10.1016/bs.mcb.2018.12.001.
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Kinetic and photonic techniques to study chemotactic signaling in sea urchin sperm.
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Sperm from sea urchins are attracted by chemical cues released by the egg-a mechanism called chemotaxis. We describe here the signaling pathway and molecular components endowing sperm with single-molecule sensitivity. Chemotactic signaling and behavioral responses occur on a timescale of a few milliseconds to seconds. We describe the techniques and chemical tools used to resolve the signaling events in time. The techniques include rapid-mixing devices, rapid stroboscopic microscopy, and photolysis of caged second messengers and chemoattractants.
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Genes referenced: LOC100887844