Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Stichopus chloronotus aqueous extract as a chondroprotective agent for human chondrocytes isolated from osteoarthitis articular cartilage in vitro.
Mohd Heikal MY
,
Ahmad Nazrun S
,
Chua KH
,
Norzana AG
.
???displayArticle.abstract???
The proinflammatory cytokines, metalloproteinases family (MMPs), inflammatory mediators PGE2, COX-2 and NO are the most important group of compounds responsible for the loss of metabolic homeostasis of articular cartilage by promoting catabolic and destructive processes in the pathogenesis of osteoarthritis (OA). Stichopus chloronotus, a marine sea cucumber which is rich in n-3 PUFAs and phenolic compound, may exert a favorable influence on the course of the disease. The objective of this study was to investigate the regeneration and anti-inflammatory potential of S. chloronotus aqueous extract (SCAE) on human OA articular chondrocytes (HOC).
METHODS: The HOC isolated from knee joint cartilage removed during surgery were cultured with SCAE for 7 days. The effect of SCAE on anabolic and catabolic gene expression was verified by real-time PCR. Monolayer chondrocytes were stained with toluidine blue whereas sGAG, NO and PGE2 production in medium were analyzed by ELISA.
RESULTS: The HOC cultured in various SCAE have polygonal morphology maintaining their chondrocytes characteristic. SAE supplementation tested was found to be effective pro-chondrogenic, anti-inflammatory and anti-oxidative agents, as evidenced by upregulation of cartilage specific markers collagen type II, aggrecan core protein and sox-9 expression and downregulation of collagen type 1, IL-1, IL-6, IL-8, MMP-1, MMP-3, MMP-13, COX-2, iNOS and PAR-2 expression. The presence of SCAE in the culture was able to increase sGAG and reduce NO and PGE2 production significantly.
CONCLUSIONS: These results suggested that SCAE demonstrated chondroprotective ability by suppressing catabolic activities, oxidative damage and effectively promoting chondrocytes growth.
Abramson,
Osteoarthritis and nitric oxide.
2008, Pubmed
Abramson,
Osteoarthritis and nitric oxide.
2008,
Pubmed
Akhtar,
Effect of a Herbal-Leucine mix on the IL-1β-induced cartilage degradation and inflammatory gene expression in human chondrocytes.
2011,
Pubmed
Amin,
Superinduction of cyclooxygenase-2 activity in human osteoarthritis-affected cartilage. Influence of nitric oxide.
1997,
Pubmed
Boileau,
Activation of proteinase-activated receptor 2 in human osteoarthritic cartilage upregulates catabolic and proinflammatory pathways capable of inducing cartilage degradation: a basic science study.
2007,
Pubmed
Cardile,
Effect of propolis on human cartilage and chondrocytes.
2003,
Pubmed
Cucchiarini,
Basic science of osteoarthritis.
2016,
Pubmed
Curtis,
Biological basis for the benefit of nutraceutical supplementation in arthritis.
2004,
Pubmed
Dai,
Silencing of microRNA-101 prevents IL-1β-induced extracellular matrix degradation in chondrocytes.
2012,
Pubmed
Dodge,
Glucosamine sulfate modulates the levels of aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chondrocytes.
2003,
Pubmed
Dozin,
Response of young, aged and osteoarthritic human articular chondrocytes to inflammatory cytokines: molecular and cellular aspects.
2002,
Pubmed
El Hajjaji,
Celecoxib has a positive effect on the overall metabolism of hyaluronan and proteoglycans in human osteoarthritic cartilage.
2003,
Pubmed
Fredalina,
Fatty acid compositions in local sea cucumber, Stichopus chloronotus, for wound healing.
1999,
Pubmed
,
Echinobase
Goldring,
Inflammation in osteoarthritis.
2011,
Pubmed
Hardy,
Cyclooxygenase 2-dependent prostaglandin E2 modulates cartilage proteoglycan degradation in human osteoarthritis explants.
2002,
Pubmed
Houard,
Homeostatic mechanisms in articular cartilage and role of inflammation in osteoarthritis.
2013,
Pubmed
Hsieh-Bonassera,
Expansion and redifferentiation of chondrocytes from osteoarthritic cartilage: cells for human cartilage tissue engineering.
2009,
Pubmed
Hurst,
Dietary fatty acids and arthritis.
2010,
Pubmed
Janakiram,
Sea Cucumbers Metabolites as Potent Anti-Cancer Agents.
2015,
Pubmed
,
Echinobase
Jerosch,
Effects of Glucosamine and Chondroitin Sulfate on Cartilage Metabolism in OA: Outlook on Other Nutrient Partners Especially Omega-3 Fatty Acids.
2011,
Pubmed
Jiménez,
Activin A/BMP2 chimera AB235 drives efficient redifferentiation of long term cultured autologous chondrocytes.
2015,
Pubmed
Kapoor,
Role of proinflammatory cytokines in the pathophysiology of osteoarthritis.
2011,
Pubmed
Knott,
Regulation of osteoarthritis by omega-3 (n-3) polyunsaturated fatty acids in a naturally occurring model of disease.
2011,
Pubmed
Lee,
A current review of molecular mechanisms regarding osteoarthritis and pain.
2013,
Pubmed
Liu,
Effects of mechanical stress on chondrocyte phenotype and chondrocyte extracellular matrix expression.
2016,
Pubmed
Maldonado,
The role of changes in extracellular matrix of cartilage in the presence of inflammation on the pathology of osteoarthritis.
2013,
Pubmed
Munirah,
Expansion of human articular chondrocytes and formation of tissue-engineered cartilage: a step towards exploring a potential use of matrix-induced cell therapy.
2010,
Pubmed
Otero,
Human chondrocyte cultures as models of cartilage-specific gene regulation.
2012,
Pubmed
Shimpo,
Regulation of prostaglandin E(2) synthesis in cells derived from chondrocytes of patients with osteoarthritis.
2009,
Pubmed
Sun,
A 3D cartilage - inflammatory cell culture system for the modeling of human osteoarthritis.
2011,
Pubmed
Tsuchida,
Cytokine profiles in the joint depend on pathology, but are different between synovial fluid, cartilage tissue and cultured chondrocytes.
2014,
Pubmed
Wang,
Interleukin-6 synthesis in human chondrocytes is regulated via the antagonistic actions of prostaglandin (PG)E2 and 15-deoxy-Δ(12,14)-PGJ2.
2011,
Pubmed
Wann,
Eicosapentaenoic acid and docosahexaenoic acid reduce interleukin-1β-mediated cartilage degradation.
2010,
Pubmed
Yin,
Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity.
2011,
Pubmed