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PLoS One
2017 Nov 10;1211:e0187333. doi: 10.1371/journal.pone.0187333.
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The axis IL-10/claudin-10 is implicated in the modulation of aggressiveness of melanoma cells by B-1 lymphocytes.
Perez EC
,
Xander P
,
Laurindo MFL
,
Novaes E Brito RR
,
Vivanco BC
,
Mortara RA
,
Mariano M
,
Lopes JD
,
Keller AC
.
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B-1 lymphocytes are known to increase the metastatic potential of B16F10 melanoma cells via the extracellular signal-regulated kinase (ERK) pathway. Since IL-10 is associated with B-1 cells performance, we hypothesized that IL-10 could be implicated in the progression of melanoma. In the present work, we found that the C57BL/6 mice, inoculated with B16F10 cells that were co-cultivated with B-1 lymphocytes from IL-10 knockout mice, developed fewer metastatic nodules than the ones which were injected with the melanoma cells that were cultivated in the presence of wild-type B-1 cells. The impairment of metastatic potential of the B16F10 cells was correlated with low activation of the ERK signaling pathway, supporting the idea that the production of IL-10 by B-1 cells influences the behavior of the tumor. A microarray analysis of the B-1 lymphocytes revealed that IL-10 deficiency is associated with down-regulation of the genes that code for claudin-10, a protein that is involved in cell-to-cell contact and that has been linked to lung adenocarcinoma. In order to determine the impact of claudin-10 in the cross-talk between B-1 lymphocytes and the B16F10 tumor cells, we took advantage of small interfering RNA. The silencing of claudin-10 gene in B-1 lymphocytes inhibited activation of the ERK pathway and abrogated the B-1-induced aggressive behavior of the B16F10 cells. Thus, our findings suggest that the axis IL-10/claudin-10 is a promising target for the development of therapeutic agents against aggressive melanoma.
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29145406
???displayArticle.pmcLink???PMC5690663 ???displayArticle.link???PLoS One
Fig 3. IL-10 deficiency is associated with impaired expression of claudin-10 in B-1 lymphocytes.Total RNA of B-1 lymphocytes enriched culture from wild-type (B-1WT) or IL-10-/- knockout (B-1IL-10-/-) mice was submitted to microarray analysis. A) Heatmap showing differential expression of eleven transcripts between B-1WT and B-1IL-10-/- lymphocytes, and the down-regulation of claudin-10 (cldn-10) in B-1IL-10-/- samples. B) Western blot analysis of cldn-10 protein in whole-cell extracts from B-1WT and B-1IL-10-/-. β-actin protein was used as internal control.
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