Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Front Chem
2017 Jan 01;5:54. doi: 10.3389/fchem.2017.00054.
Show Gene links
Show Anatomy links
Analysis of Urine Composition in Type II Diabetic Mice after Intervention Therapy Using Holothurian Polypeptides.
Li Y
,
Xu J
,
Su X
.
???displayArticle.abstract???
Hydrolysates and peptide fractions (PF) obtained from sea cucumber with commercial enzyme were studied on the hyperglycemic and renal protective effects on db/db rats using urine metabolomics. Compared with the control group the polypeptides from the two species could significantly reduce the urine glucose and urea. We also tried to address the compositions of highly expressed urinary proteins using a proteomics approach. They were serum albumins, AMBP proteins, negative trypsin, elastase, and urinary protein, GAPDH, a receptor of urokinase-type plasminogen activator (uPAR), and Ig kappa chain C region. We used the electronic nose to quickly detect changes in the volatile substances in mice urine after holothurian polypeptides (HPP) fed, and the results show it can identify the difference between treatment groups with the control group without overlapping. The protein express mechanism of HPP treating diabetes was discussed, and we suggested these two peptides with the hypoglycemic and renal protective activity might be utilized as nutraceuticals.
Figure 1. Two-dimensional electrophoregram. C, Control group; M, model group; Me, positive drug group; A, A. molpadioides's polypeptide group; S, A. japonicus's polypeptide group.
Figure 2. Odor analysis of urine samples of db/db mice using electronic nose. C, Control group; M, model group; Me, positive drug group; A, A. molpadioides's polypeptide group; S, A. japonicus's polypeptide group. (Top) PCA; (Bottom) LDA.
Figure 3. Volatile compounds in the urine of db/db mice. C, Control group; M, model group; Me, positive drug group; A, A. molpadioides's polypeptide group; S, A. japonicus's polypeptide group.
Figure 4. Contents of metabolites in the urine of db/db mice. C, Control group; M, model group; Me, positive drug group; A, A. molpadioides's polypeptide group; S, A. japonicus's polypeptide group. I, Analysis of intersection of metabolites in each group; II, Alcohols; III, Amines. Additives; IV Organic acids; V, Sugar; VI, Amino acids; VII, Amino acid derivatives; VIII, Ammoniums; IX, Nucleic acids; X, Vitamins; XI, Additives; XII, Ketones.
Adrian,
Hypotrypsinaemia in diabetes mellitus.
1979, Pubmed
Adrian,
Hypotrypsinaemia in diabetes mellitus.
1979,
Pubmed
Beckonert,
Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts.
2007,
Pubmed
Bingol,
NMR/MS Translator for the Enhanced Simultaneous Analysis of Metabolomics Mixtures by NMR Spectroscopy and Mass Spectrometry: Application to Human Urine.
2015,
Pubmed
Bordbar,
High-value components and bioactives from sea cucumbers for functional foods--a review.
2011,
Pubmed
,
Echinobase
Brownlee,
Biochemistry and molecular cell biology of diabetic complications.
2001,
Pubmed
Brownlee,
Measurement of glycosylated amino acids and peptides from urine of diabetic patients using affinity chromatography.
1980,
Pubmed
Bruce,
Investigation of human blood plasma sample preparation for performing metabolomics using ultrahigh performance liquid chromatography/mass spectrometry.
2009,
Pubmed
Elliott,
Substantial reductions in the number of diabetic ketoacidosis and severe hypoglycaemia episodes requiring emergency treatment lead to reduced costs after structured education in adults with Type 1 diabetes.
2014,
Pubmed
Forbes,
Mechanisms of diabetic complications.
2013,
Pubmed
Fu,
Discovery and verification of urinary peptides in type 2 diabetes mellitus with kidney injury.
2016,
Pubmed
Good,
Naturally occurring human urinary peptides for use in diagnosis of chronic kidney disease.
2010,
Pubmed
Khamlichi,
Primary structure of a monoclonal kappa chain in myeloma with light chain deposition disease.
1992,
Pubmed
Klein,
The role of urinary peptidomics in kidney disease research.
2016,
Pubmed
Kraus,
Association of Plasma Small-Molecule Intermediate Metabolites With Age and Body Mass Index Across Six Diverse Study Populations.
2016,
Pubmed
Lebovitz,
Diabetic ketoacidosis.
1995,
Pubmed
McCullough,
Acute renal failure after coronary intervention: incidence, risk factors, and relationship to mortality.
1997,
Pubmed
Modena,
Changes in Urinary Microbiome Populations Correlate in Kidney Transplants With Interstitial Fibrosis and Tubular Atrophy Documented in Early Surveillance Biopsies.
2017,
Pubmed
Morris,
Adherence to insulin treatment, glycaemic control, and ketoacidosis in insulin-dependent diabetes mellitus. The DARTS/MEMO Collaboration. Diabetes Audit and Research in Tayside Scotland. Medicines Monitoring Unit.
1997,
Pubmed
Papadopoulos,
Omics databases on kidney disease: where they can be found and how to benefit from them.
2016,
Pubmed
Rosolowsky,
Risk for ESRD in type 1 diabetes remains high despite renoprotection.
2011,
Pubmed
Rothman,
Glutamate and the pathophysiology of hypoxic--ischemic brain damage.
1986,
Pubmed
Shaw,
Global estimates of the prevalence of diabetes for 2010 and 2030.
2010,
Pubmed
Stec,
Alterations of urinary metabolite profile in model diabetic nephropathy.
2015,
Pubmed
Tabák,
Prediabetes: a high-risk state for diabetes development.
2012,
Pubmed
Vasir,
Effects of diabetes and hypoxia on gene markers of angiogenesis (HGF, cMET, uPA and uPAR, TGF-alpha, TGF-beta, bFGF and Vimentin) in cultured and transplanted rat islets.
2000,
Pubmed
Vieira,
Structure of a fucose-branched chondroitin sulfate from sea cucumber. Evidence for the presence of 3-O-sulfo-beta-D-glucuronosyl residues.
1991,
Pubmed
,
Echinobase
Vieira,
Occurrence of a unique fucose-branched chondroitin sulfate in the body wall of a sea cucumber.
1988,
Pubmed
,
Echinobase
Wei,
Circulating urokinase receptor as a cause of focal segmental glomerulosclerosis.
2011,
Pubmed
Weissinger,
Urinary proteomics employing capillary electrophoresis coupled to mass spectrometry in the monitoring of patients after stem cell transplantation.
2014,
Pubmed
Wu,
Structure and effect of sulfated fucose branches on anticoagulant activity of the fucosylated chondroitin sulfate from sea cucumber Thelenata ananas.
2012,
Pubmed
,
Echinobase
You,
Antioxidant and antiproliferative activities of loach ( Misgurnus anguillicaudatus ) peptides prepared by papain digestion.
2011,
Pubmed
