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Mar Biotechnol (NY)
2016 Jun 01;183:384-95. doi: 10.1007/s10126-016-9701-0.
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Characterization of an Alpha Type Carbonic Anhydrase from Paracentrotus lividus Sea Urchin Embryos.
Karakostis K
,
Costa C
,
Zito F
,
Brümmer F
,
Matranga V
.
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Carbonic anhydrases (CA) are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. In the sea urchin, CA has a role in the formation of the calcitic skeleton during embryo development. Here, we report a newly identified mRNA sequence from embryos of the sea urchin Paracentrotus lividus, referred to as Pl-can. The complete coding sequence was identified with the aid of both EST databases and experimental procedures. Pl-CAN is a 447 aa-long protein, with an estimated molecular mass of 48.5 kDa and an isoelectric point of 6.83. The in silico study of functional domains showed, in addition to the alpha type CA-specific domain, the presence of an unexpected glycine-rich region at the N-terminal of the molecule. This is not found in any other species described so far, but probably it is restricted to the sea urchins. The phylogenetic analysis indicated that Pl-CAN is evolutionarily closer to human among chordates than to other species. The putative role(s) of the identified domains is discussed. The Pl-can temporal and spatial expression profiles, analyzed throughout embryo development by comparative qPCR and whole-mount in situ hybridization (WMISH), showed that Pl-can mRNA is specifically expressed in the primary mesenchyme cells (PMC) of the embryo and levels increase along with the growth of the embryonic skeleton, reaching a peak at the pluteus stage. A recombinant fusion protein was produced in E. coli and used to raise specific antibodies in mice recognized the endogenous Pl-CAN by Western blot in embryo extracts from gastrula and pluteus.
Addadi,
Mollusk shell formation: a source of new concepts for understanding biomineralization processes.
2006, Pubmed
Addadi,
Mollusk shell formation: a source of new concepts for understanding biomineralization processes.
2006,
Pubmed
Adomako-Ankomah,
P58-A and P58-B: novel proteins that mediate skeletogenesis in the sea urchin embryo.
2011,
Pubmed
,
Echinobase
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Amata,
Catalytic activity of a ζ-class zinc and cadmium containing carbonic anhydrase. Compared work mechanisms.
2011,
Pubmed
Arnett,
Autoantibodies to fibrillarin in systemic sclerosis (scleroderma). An immunogenetic, serologic, and clinical analysis.
1996,
Pubmed
Barneche,
Fibrillarin genes encode both a conserved nucleolar protein and a novel small nucleolar RNA involved in ribosomal RNA methylation in Arabidopsis thaliana.
2000,
Pubmed
Bendtsen,
Improved prediction of signal peptides: SignalP 3.0.
2004,
Pubmed
Blom,
Sequence and structure-based prediction of eukaryotic protein phosphorylation sites.
1999,
Pubmed
Bonaventura,
Stress response gene activation protects sea urchin embryos exposed to X-rays.
2011,
Pubmed
,
Echinobase
Boone,
Carbonic anhydrases and their biotechnological applications.
2013,
Pubmed
Cannon,
Carboxysomal carbonic anhydrases: Structure and role in microbial CO2 fixation.
2010,
Pubmed
Capasso,
An overview of the alpha-, beta- and gamma-carbonic anhydrases from Bacteria: can bacterial carbonic anhydrases shed new light on evolution of bacteria?
2015,
Pubmed
Chow,
Carbonic anhydrase activity in developing sea urchin embryos.
1979,
Pubmed
,
Echinobase
Costa,
Phylogenetic analysis and homology modelling of Paracentrotus lividus nectin.
2010,
Pubmed
,
Echinobase
Costa,
Phylogenetic analysis and expression patterns of p16 and p19 in Paracentrotus lividus embryos.
2012,
Pubmed
,
Echinobase
Cross,
Delivering proteins for export from the cytosol.
2009,
Pubmed
Edgar,
MUSCLE: multiple sequence alignment with high accuracy and high throughput.
2004,
Pubmed
Ettensohn,
Lessons from a gene regulatory network: echinoderm skeletogenesis provides insights into evolution, plasticity and morphogenesis.
2009,
Pubmed
,
Echinobase
Frigeri,
Identification of proteins from a cell wall fraction of the diatom Thalassiosira pseudonana: insights into silica structure formation.
2006,
Pubmed
Giordano,
CO2 concentrating mechanisms in algae: mechanisms, environmental modulation, and evolution.
2005,
Pubmed
Guss,
Skeletal morphogenesis in the sea urchin embryo: regulation of primary mesenchyme gene expression and skeletal rod growth by ectoderm-derived cues.
1997,
Pubmed
,
Echinobase
Hodor,
The dynamics and regulation of mesenchymal cell fusion in the sea urchin embryo.
1998,
Pubmed
,
Echinobase
Jackson,
A horizontal gene transfer supported the evolution of an early metazoan biomineralization strategy.
2011,
Pubmed
Kakei,
Aspects of carbonic anhydrase and carbonate content during mineralization of the rat enamel.
1996,
Pubmed
Karakostis,
A minimal molecular toolkit for mineral deposition? Biochemistry and proteomics of the test matrix of adult specimens of the sea urchin Paracentrotus lividus.
2016,
Pubmed
,
Echinobase
Lehenkari,
Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+.
1998,
Pubmed
Livak,
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
2001,
Pubmed
Livingston,
A genome-wide analysis of biomineralization-related proteins in the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Love,
Gene expression patterns in a novel animal appendage: the sea urchin pluteus arm.
2007,
Pubmed
,
Echinobase
Mangeon,
Functional diversity of the plant glycine-rich proteins superfamily.
2010,
Pubmed
Mann,
Proteomic analysis of sea urchin (Strongylocentrotus purpuratus) spicule matrix.
2010,
Pubmed
,
Echinobase
Mann,
The sea urchin (Strongylocentrotus purpuratus) test and spine proteomes.
2008,
Pubmed
,
Echinobase
Mann,
In-depth, high-accuracy proteomics of sea urchin tooth organic matrix.
2008,
Pubmed
,
Echinobase
Matranga,
Echinoderms as blueprints for biocalcification: regulation of skeletogenic genes and matrices.
2011,
Pubmed
,
Echinobase
Mitsunaga,
Carbonic anhydrase activity in developing sea urchin embryos with special reference to calcification of spicules.
1986,
Pubmed
,
Echinobase
Miyamoto,
A carbonic anhydrase from the nacreous layer in oyster pearls.
1996,
Pubmed
Müller,
Common genetic denominators for Ca++-based skeleton in Metazoa: role of osteoclast-stimulating factor and of carbonic anhydrase in a calcareous sponge.
2012,
Pubmed
Okazaki,
Skeleton formation of sea urchin larvae. V. Continuous observation of the process of matrix formation.
1965,
Pubmed
,
Echinobase
Pamirsky,
Origin and status of homologous proteins of biomineralization (biosilicification) in the taxonomy of phylogenetic domains.
2013,
Pubmed
Pinsino,
Sea urchin embryos as an in vivo model for the assessment of manganese toxicity: developmental and stress response effects.
2010,
Pubmed
,
Echinobase
Politi,
Transformation mechanism of amorphous calcium carbonate into calcite in the sea urchin larval spicule.
2008,
Pubmed
,
Echinobase
Rafiq,
The genomic regulatory control of skeletal morphogenesis in the sea urchin.
2012,
Pubmed
,
Echinobase
Rapoport,
Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes.
2007,
Pubmed
Russo,
The newly characterized Pl-jun is specifically expressed in skeletogenic cells of the Paracentrotus lividus sea urchin embryo.
2014,
Pubmed
,
Echinobase
Russo,
Transcriptional increase and misexpression of 14-3-3 epsilon in sea urchin embryos exposed to UV-B.
2010,
Pubmed
,
Echinobase
Saito,
Structure of bovine carbonic anhydrase II at 1.95 A resolution.
2004,
Pubmed
Salem,
Cloning of the calpain regulatory subunit cDNA from fish reveals a divergent domain-V.
2004,
Pubmed
Satoh,
Inhibition of photosynthesis by intracellular carbonic anhydrase in microalgae under excess concentrations of CO(2).
2001,
Pubmed
Shi,
Bicarbonate/CO2 is not required for zona pellucida- or progesterone-induced acrosomal exocytosis of mouse spermatozoa but is essential for capacitation.
1995,
Pubmed
Shibata,
Genes essential to sodium-dependent bicarbonate transport in cyanobacteria: function and phylogenetic analysis.
2002,
Pubmed
Smith,
Prokaryotic carbonic anhydrases.
2000,
Pubmed
So,
A novel evolutionary lineage of carbonic anhydrase (epsilon class) is a component of the carboxysome shell.
2004,
Pubmed
Song,
Identification two novel nacrein-like proteins involved in the shell formation of the Pacific oyster Crassostrea gigas.
2014,
Pubmed
Stumpp,
Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification.
2012,
Pubmed
,
Echinobase
Supuran,
Carbonic anhydrases--an overview.
2008,
Pubmed
Tamura,
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
2011,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Tohse,
Effects of enzyme and anion transport inhibitors on in vitro incorporation of inorganic carbon and calcium into endolymph and otoliths in salmon Oncorhynchus masou.
2001,
Pubmed
Veis,
Organic matrix-related mineralization of sea urchin spicules, spines, test and teeth.
2011,
Pubmed
,
Echinobase
Vidavsky,
Initial stages of calcium uptake and mineral deposition in sea urchin embryos.
2014,
Pubmed
,
Echinobase
Wang,
Silicateins, silicatein interactors and cellular interplay in sponge skeletogenesis: formation of glass fiber-like spicules.
2012,
Pubmed
Westbroek,
A marriage of bone and nacre.
1998,
Pubmed
Wilt,
Biomineralization of the spicules of sea urchin embryos.
2002,
Pubmed
,
Echinobase
Wilt,
Developmental biology meets materials science: Morphogenesis of biomineralized structures.
2005,
Pubmed
,
Echinobase
Xiang,
Amorphous calcium carbonate precipitation by cellular biomineralization in mantle cell cultures of Pinctada fucata.
2014,
Pubmed
Zito,
Carbonic anhydrase inhibition blocks skeletogenesis and echinochrome production in Paracentrotus lividus and Heliocidaris tuberculata embryos and larvae.
2015,
Pubmed
,
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