Sampath K , Robertson EJ .

Biotechnology and Biological Sciences Research Council

	Figure 1. Schematic of maternal sqt/nodal RNA expression in early zebrafish embryos. Sqt RNA localizes by the four-cell stage in one or two cells, which later form dorsal progenitors, marked by nuclear β-catenin and gsc expression.
	Figure 2. Schematic of sqt DLE sequence and structure motif. (a) Schematic of sqt exon 3 and the 3′-UTR, showing the dorsal localization element (DLE; green shading), which includes a single-stranded motif (pink box) and a short stem-loop structure. (b) The sequence of the single-stranded motif (pink highlighted region) and the stem-loop structure of the hairpin are shown.
	Figure 3. Schematic of zebrafish ybx1 and sqt/nodal mutant phenotypes. In sqt mutants gsc expression is initiated but not maintained, whereas in Mybx1 mutants, sqt RNA is mis-localized and gsc is expressed prematurely in the expanded yolk syncytial layer (YSL). DIC images show the phenotypes of wild-type or mutant embryos.
	Figure 4. Model of translational repression of Nodal pathway components. (a) In early zebrafish embryos, Ybx1 represses squint/nodal translation by binding to the translation pre-initiation complex proteins and the squint DLE (solid blue box). Squint/Nodal translation is activated from late blastula stages by unknown factors. (b) In vegetal cells of frog embryos, Bic-C represses xCR1 translation by binding to the translation pre-initiation complex proteins and the xCR1 TCE (solid green box). In animal cap cells, xCR1 translation is activated in the absence of Bic-C. (c) miRNA-378a-5p (black comb) binds to the 3′-UTR (solid red box) of human Nodal RNA and unknown factors (?) to repress Nodal translation in the human placenta. In the absence of miRNA-378a-5p, nodal translation is activated.

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