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Echinobase
ECB-ART-43719
PLoS One 2014 Dec 01;912:e113866. doi: 10.1371/journal.pone.0113866.
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Manipulation of developing juvenile structures in purple sea urchins (Strongylocentrotus purpuratus) by morpholino injection into late stage larvae.

Heyland A , Hodin J , Bishop C .


Abstract
Sea urchins have been used as experimental organisms for developmental biology for over a century. Yet, as is the case for many other marine invertebrates, understanding the development of the juveniles and adults has lagged far behind that of their embryos and larvae. The reasons for this are, in large part, due to the difficulty of experimentally manipulating juvenile development. Here we develop and validate a technique for injecting compounds into juvenile rudiments of the purple sea urchin, Strongylocentrotus purpuratus. We first document the distribution of rhodaminated dextran injected into different compartments of the juvenile rudiment of sea urchin larvae. Then, to test the potential of this technique to manipulate development, we injected Vivo-Morpholinos (vMOs) designed to knock down p58b and p16, two proteins involved in the elongation of S. purpuratus larval skeleton. Rudiments injected with these vMOs showed a delay in the growth of some juvenile skeletal elements relative to controls. These data provide the first evidence that vMOs, which are designed to cross cell membranes, can be used to transiently manipulate gene function in later developmental stages in sea urchins. We therefore propose that injection of vMOs into juvenile rudiments, as shown here, is a viable approach to testing hypotheses about gene function during development, including metamorphosis.

PubMed ID: 25436992
PMC ID: PMC4250057
Article link: PLoS One


Species referenced: Echinodermata
Genes referenced: adcy10 LOC100887844 LOC100893907 LOC115919910 LOC115925415 P16
Morpholinos: P16 MO1 p58b MO1


Article Images: [+] show captions
References [+] :
Adomako-Ankomah, P58-A and P58-B: novel proteins that mediate skeletogenesis in the sea urchin embryo. 2011, Pubmed, Echinobase