Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Cytoskeleton (Hoboken)
2014 Aug 01;718:484-500. doi: 10.1002/cm.21186.
Show Gene links
Show Anatomy links
Broadening the spectrum of actin-based protrusive activity mediated by Arp2/3 complex-facilitated polymerization: motility of cytoplasmic ridges and tubular projections.
Henson JH
,
Gianakas AD
,
Henson LH
,
Lakin CL
,
Voss MK
,
Bewersdorf J
,
Oldenbourg R
,
Morris RL
.
???displayArticle.abstract??? Arp2/3 complex-facilitated actin polymerization plays an essential role in a variety of cellular functions including motility, adherence, endocytosis, and trafficking. In the present study, we employ the sea urchin coelomocyte experimental model system to test the hypotheses that Arp2/3 complex-nucleated actin assembly mediates the motility of two unusual cellular protrusions; the cytoplasmic ridges present during coelomocyte spreading, and inducible, tubular-shaped, and neurite-like projections. Our investigations couple pharmacological manipulation employing inhibitors of actin polymerization and the Arp2/3 complex with a wide array of imaging methods including digitally enhanced phase contrast, DIC, and polarization light microscopy of live cells; conventional, confocal and super-resolution light microscopy of fluorescently labeled cells; and scanning and transmission electron microscopy. Taken together, the results of this study indicate that Arp2/3 complex-facilitated actin polymerization underlies the motility of coelomocyte cytoplasmic ridges and tubular projections, that these processes are related to each other, and that they have been preliminarily identified in other cell types. The results also highlight the broad spectrum of actin-based protrusive activities dependent on the Arp2/3 complex and provide additional insights into the pervasive nature of this ubiquitous actin nucleator. Furthermore, we provide the first evidence of a possible mechanistic difference between the impacts of the small molecule drugs BDM and CK666 on the Arp2/3 complex.
Bear,
Ena/VASP: towards resolving a pointed controversy at the barbed end.
2009, Pubmed
Bear,
Ena/VASP: towards resolving a pointed controversy at the barbed end.
2009,
Pubmed
Danilchik,
Intrinsic chiral properties of the Xenopus egg cortex: an early indicator of left-right asymmetry?
2006,
Pubmed
Dent,
Axon branching requires interactions between dynamic microtubules and actin filaments.
2001,
Pubmed
Edds,
Isolation and characterization of two forms of a cytoskeleton.
1979,
Pubmed
,
Echinobase
Firat-Karalar,
New mechanisms and functions of actin nucleation.
2011,
Pubmed
Forscher,
Novel form of growth cone motility involving site-directed actin filament assembly.
1992,
Pubmed
Gross,
Echinoderm immunity and the evolution of the complement system.
1999,
Pubmed
,
Echinobase
Gupton,
Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin.
2005,
Pubmed
Gupton,
mDia2 regulates actin and focal adhesion dynamics and organization in the lamella for efficient epithelial cell migration.
2007,
Pubmed
Henson,
Two components of actin-based retrograde flow in sea urchin coelomocytes.
1999,
Pubmed
,
Echinobase
Henson,
Wound closure in the lamellipodia of single cells: mediation by actin polymerization in the absence of an actomyosin purse string.
2002,
Pubmed
,
Echinobase
Henson,
Actin-based centripetal flow: phosphatase inhibition by calyculin-A alters flow pattern, actin organization, and actomyosin distribution.
2003,
Pubmed
,
Echinobase
Henson,
Structure and dynamics of an Arp2/3 complex-independent component of the lamellipodial actin network.
2009,
Pubmed
,
Echinobase
Johnston,
Arp2/3 complex activity in filopodia of spreading cells.
2008,
Pubmed
Katoh,
Birefringence imaging directly reveals architectural dynamics of filamentous actin in living growth cones.
1999,
Pubmed
Koestler,
Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin.
2013,
Pubmed
Korobova,
Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.
2008,
Pubmed
Korobova,
Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis.
2010,
Pubmed
Nicholson-Dykstra,
Arp2 depletion inhibits sheet-like protrusions but not linear protrusions of fibroblasts and lymphocytes.
2008,
Pubmed
Nolen,
Characterization of two classes of small molecule inhibitors of Arp2/3 complex.
2009,
Pubmed
Onesto,
Characterization of EHT 1864, a novel small molecule inhibitor of Rac family small GTPases.
2008,
Pubmed
Pollard,
Regulation of actin filament assembly by Arp2/3 complex and formins.
2007,
Pubmed
Pollard,
Actin, a central player in cell shape and movement.
2009,
Pubmed
Ponti,
Two distinct actin networks drive the protrusion of migrating cells.
2004,
Pubmed
Ridley,
Life at the leading edge.
2011,
Pubmed
Rochlin,
Polymerizing microtubules activate site-directed F-actin assembly in nerve growth cones.
1999,
Pubmed
Rotty,
New insights into the regulation and cellular functions of the ARP2/3 complex.
2013,
Pubmed
Schaefer,
Filopodia and actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cones.
2002,
Pubmed
Schafer,
Visualization and molecular analysis of actin assembly in living cells.
1998,
Pubmed
Smith,
Echinoderm immunity.
2010,
Pubmed
,
Echinobase
Spillane,
The actin nucleating Arp2/3 complex contributes to the formation of axonal filopodia and branches through the regulation of actin patch precursors to filopodia.
2011,
Pubmed
Strasser,
Arp2/3 is a negative regulator of growth cone translocation.
2004,
Pubmed
Svitkina,
Mechanism of filopodia initiation by reorganization of a dendritic network.
2003,
Pubmed
Svitkina,
Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation.
1997,
Pubmed
Svitkina,
Electron microscopic analysis of the leading edge in migrating cells.
2007,
Pubmed
Svitkina,
Ultrastructure of protrusive actin filament arrays.
2013,
Pubmed
Vitriol,
Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins.
2007,
Pubmed
Yang,
Arp2/3 complex-dependent actin networks constrain myosin II function in driving retrograde actin flow.
2012,
Pubmed
Yang,
Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells.
2007,
Pubmed
Yang,
Filopodia initiation: focus on the Arp2/3 complex and formins.
2011,
Pubmed
Yarrow,
Rapid de-localization of actin leading edge components with BDM treatment.
2003,
Pubmed
