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Development
2014 Aug 01;14116:3134-42. doi: 10.1242/dev.110395.
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Deadenylase depletion protects inherited mRNAs in primordial germ cells.
Swartz SZ
,
Reich AM
,
Oulhen N
,
Raz T
,
Milos PM
,
Campanale JP
,
Hamdoun A
,
Wessel GM
.
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A crucial event in animal development is the specification of primordial germ cells (PGCs), which become the stem cells that create sperm and eggs. How PGCs are created provides a valuable paradigm for understanding stem cells in general. We find that the PGCs of the sea urchin Strongylocentrotus purpuratus exhibit broad transcriptional repression, yet enrichment for a set of inherited mRNAs. Enrichment of several germline determinants in the PGCs requires the RNA-binding protein Nanos to target the transcript that encodes CNOT6, a deadenylase, for degradation in the PGCs, thereby creating a stable environment for RNA. Misexpression of CNOT6 in the PGCs results in their failure to retain Seawi transcripts and Vasa protein. Conversely, broad knockdown of CNOT6 expands the domain of Seawi RNA as well as exogenous reporters. Thus, Nanos-dependent spatially restricted CNOT6 differential expression is used to selectively localize germline RNAs to the PGCs. Our findings support a ''time capsule'' model of germline determination, whereby the PGCs are insulated from differentiation by retaining the molecular characteristics of the totipotent egg and early embryo.
Bashirullah,
Joint action of two RNA degradation pathways controls the timing of maternal transcript elimination at the midblastula transition in Drosophila melanogaster.
1999, Pubmed
Bashirullah,
Joint action of two RNA degradation pathways controls the timing of maternal transcript elimination at the midblastula transition in Drosophila melanogaster.
1999,
Pubmed
Bushati,
Temporal reciprocity of miRNAs and their targets during the maternal-to-zygotic transition in Drosophila.
2008,
Pubmed
Campanale,
Programmed reduction of ABC transporter activity in sea urchin germline progenitors.
2012,
Pubmed
,
Echinobase
Chen,
Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.
2012,
Pubmed
Collart,
The Ccr4--not complex.
2012,
Pubmed
Conesa,
Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research.
2005,
Pubmed
Ewen-Campen,
The molecular machinery of germ line specification.
2010,
Pubmed
Extavour,
Evolution of the bilaterian germ line: lineage origin and modulation of specification mechanisms.
2007,
Pubmed
Extavour,
Mechanisms of germ cell specification across the metazoans: epigenesis and preformation.
2003,
Pubmed
Gerber,
Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.
2006,
Pubmed
Giraldez,
Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs.
2006,
Pubmed
Gustafson,
Exogenous RNA is selectively retained in the small micromeres during sea urchin embryogenesis.
2010,
Pubmed
,
Echinobase
Gustafson,
Post-translational regulation by gustavus contributes to selective Vasa protein accumulation in multipotent cells during embryogenesis.
2011,
Pubmed
,
Echinobase
Gökirmak,
Localization and substrate selectivity of sea urchin multidrug (MDR) efflux transporters.
2012,
Pubmed
,
Echinobase
Juliano,
Nanos functions to maintain the fate of the small micromere lineage in the sea urchin embryo.
2010,
Pubmed
,
Echinobase
Juliano,
Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.
2006,
Pubmed
,
Echinobase
Kedde,
RNA-binding protein Dnd1 inhibits microRNA access to target mRNA.
2007,
Pubmed
Le Borgne,
Notch signaling: endocytosis makes delta signal better.
2003,
Pubmed
Lessard,
Chromatin regulatory mechanisms in pluripotency.
2010,
Pubmed
Lipson,
Quantification of the yeast transcriptome by single-molecule sequencing.
2009,
Pubmed
Miller,
Characterization of the role of cadherin in regulating cell adhesion during sea urchin development.
1997,
Pubmed
,
Echinobase
Mishima,
Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430.
2006,
Pubmed
Nakamura,
Less is more: specification of the germline by transcriptional repression.
2008,
Pubmed
Oliveri,
A regulatory gene network that directs micromere specification in the sea urchin embryo.
2002,
Pubmed
,
Echinobase
Oulhen,
Retention of exogenous mRNAs selectively in the germ cells of the sea urchin requires only a 5'-cap and a 3'-UTR.
2013,
Pubmed
,
Echinobase
Pehrson,
The fate of the small micromeres in sea urchin development.
1986,
Pubmed
,
Echinobase
Rouget,
Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo.
2010,
Pubmed
Schulz,
Oases: robust de novo RNA-seq assembly across the dynamic range of expression levels.
2012,
Pubmed
Semotok,
Smaug recruits the CCR4/POP2/NOT deadenylase complex to trigger maternal transcript localization in the early Drosophila embryo.
2005,
Pubmed
Seydoux,
Pathway to totipotency: lessons from germ cells.
2006,
Pubmed
Seydoux,
Transcriptionally repressed germ cells lack a subpopulation of phosphorylated RNA polymerase II in early embryos of Caenorhabditis elegans and Drosophila melanogaster.
1997,
Pubmed
Shirae-Kurabayashi,
Ci-Pem-1 localizes to the nucleus and represses somatic gene transcription in the germline of Ciona intestinalis embryos.
2011,
Pubmed
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Song,
Select microRNAs are essential for early development in the sea urchin.
2012,
Pubmed
,
Echinobase
Suzuki,
Interaction between NANOS2 and the CCR4-NOT deadenylation complex is essential for male germ cell development in mouse.
2012,
Pubmed
Takeda,
DAZL relieves miRNA-mediated repression of germline mRNAs by controlling poly(A) tail length in zebrafish.
2009,
Pubmed
Tanaka,
Study of the Lineage and Cell Cycle of Small Micromeres in Embryos of the Sea Urchin, Hemicentrotus pulcherrimus: (small micromeres/cell cycle/cell lineage/unequal cleavage/sea urchin).
1990,
Pubmed
,
Echinobase
Van Etten,
Human Pumilio proteins recruit multiple deadenylases to efficiently repress messenger RNAs.
2012,
Pubmed
Voronina,
Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development.
2008,
Pubmed
,
Echinobase
Wei,
A database of mRNA expression patterns for the sea urchin embryo.
2006,
Pubmed
,
Echinobase
Weidmann,
Drosophila Pumilio protein contains multiple autonomous repression domains that regulate mRNAs independently of Nanos and brain tumor.
2012,
Pubmed
White,
PUM2, a novel murine puf protein, and its consensus RNA-binding site.
2001,
Pubmed
Wreden,
Nanos and pumilio establish embryonic polarity in Drosophila by promoting posterior deadenylation of hunchback mRNA.
1997,
Pubmed
Yajima,
Piwi regulates Vasa accumulation during embryogenesis in the sea urchin.
2014,
Pubmed
,
Echinobase
Yajima,
Small micromeres contribute to the germline in the sea urchin.
2011,
Pubmed
,
Echinobase
Yajima,
Autonomy in specification of primordial germ cells and their passive translocation in the sea urchin.
2012,
Pubmed
,
Echinobase
Zaessinger,
Oskar allows nanos mRNA translation in Drosophila embryos by preventing its deadenylation by Smaug/CCR4.
2006,
Pubmed
