Biochem Biophys Res Commun 2014 Aug 01;4503:1135-41. doi: 10.1016/j.bbrc.2014.05.098.

Conservation of sequence and function in fertilization of the cortical granule serine protease in echinoderms.

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Conservation of the cortical granule serine protease during fertilization in echinoderms was tested both functionally in sea stars, and computationally throughout the echinoderm phylum. We find that the inhibitor of serine protease (soybean trypsin inhibitor) effectively blocks proper transition of the sea star fertilization envelope into a protective sperm repellent, whereas inhibitors of the other main types of proteases had no effect. Scanning the transcriptomes of 15 different echinoderm ovaries revealed sequences of high conservation to the originally identified sea urchin cortical serine protease, CGSP1. These conserved sequences contained the catalytic triad necessary for enzymatic activity, and the tandemly repeated LDLr-like repeats. We conclude that the protease involved in the slow block to polyspermy is an essential and conserved element of fertilization in echinoderms, and may provide an important reagent for identification and testing of the cell surface proteins in eggs necessary for sperm binding.

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Genes referenced: cgsp1 LOC100887844 LOC115919910 LOC578116 LOC582875 LOC752081 LOC756768

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