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Methods Mol Biol 2014 Jan 01;1128:249-62. doi: 10.1007/978-1-62703-974-1_17.
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Multicolor labeling in developmental gene regulatory network analysis.

Sethi AJ , Angerer RC , Angerer LM .

The sea urchin embryo is an important model system for developmental gene regulatory network (GRN) analysis. This chapter describes the use of multicolor fluorescent in situ hybridization (FISH) as well as a combination of FISH and immunohistochemistry in sea urchin embryonic GRN studies. The methods presented here can be applied to a variety of experimental settings where accurate spatial resolution of multiple gene products is required for constructing a developmental GRN.

PubMed ID: 24567220
PMC ID: PMC4609530
Article link: Methods Mol Biol
Grant support: [+]

Genes referenced: LOC100887844 LOC575170

References [+] :
Davidson, Properties of developmental gene regulatory networks. 2009, Pubmed, Echinobase