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Elife 2013 Sep 10;2:e00683. doi: 10.7554/eLife.00683.
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An essential role for maternal control of Nodal signaling.

Kumari P , Gilligan PC , Lim S , Tran LD , Winkler S , Philp R , Sampath K .

Growth factor signaling is essential for pattern formation, growth, differentiation, and maintenance of stem cell pluripotency. Nodal-related signaling factors are required for axis formation and germ layer specification from sea urchins to mammals. Maternal transcripts of the zebrafish Nodal factor, Squint (Sqt), are localized to future embryonic dorsal. The mechanisms by which maternal sqt/nodal RNA is localized and regulated have been unclear. Here, we show that maternal control of Nodal signaling via the conserved Y box-binding protein 1 (Ybx1) is essential. We identified Ybx1 via a proteomic screen. Ybx1 recognizes the 3'' untranslated region (UTR) of sqt RNA and prevents premature translation and Sqt/Nodal signaling. Maternal-effect mutations in zebrafish ybx1 lead to deregulated Nodal signaling, gastrulation failure, and embryonic lethality. Implanted Nodal-coated beads phenocopy ybx1 mutant defects. Thus, Ybx1 prevents ectopic Nodal activity, revealing a new paradigm in the regulation of Nodal signaling, which is likely to be conserved. DOI:

PubMed ID: 24040511
PMC ID: PMC3771576
Article link: Elife

Species referenced: Echinodermata
Genes referenced: baz1b ddx4 fgf gscl lefty1 LOC100887844 LOC100891456 LOC100893798 LOC100893907 LOC115919910 LOC574674 LOC590297 LOC593824 LOC593945 LOC594236 LOC594261 nodall tubgcp2 wnt8a

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References [+] :
Bally-Cuif, Characterization of the zebrafish Orb/CPEB-related RNA binding protein and localization of maternal components in the zebrafish oocyte. 1999, Pubmed