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Mob Genet Elements
2012 Sep 01;25:244-247. doi: 10.4161/mge.22644.
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Retrotransposons in animal regeneration: Overlooked components of the regenerative machinery?
Mashanov VS
,
Zueva OR
,
García-Arrarás JE
.
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Research on the involvement of retroelements in developmental processes has been gaining momentum recently; however, most of the studies published so far have been focused on embryonic development. This commentary presents two recent papers, which document significant changes in transcriptional activity of retroelements in two different model systems, salamander limb regeneration and regeneration of radial organs in the sea cucumber Holothuria glaberrima. We hypothesize that transcriptional activity of the retrotransposons can be specifically controlled by the host and may play some hitherto unrecognized role in regeneration.
Figure 1. (A) The model organism, Holothuria glaberrima Selenka, 1867 (Echinodermata: Holothuroidea). (B) Injury paradigm. One of the five radial organ complexes (including the radial nerve, coelomic canal and muscle) was cut at about the mid-body level. For clarity, the diagram shows only the nervous system. The injured radial nerve is shaded in red.
Figure 2. Reverse transcription polymerase chain reaction (RT-PCR) showing temporal expression of Gypsy-1_Hg and Gypsy-2_Hg at different time points of visceral regeneration (Day 3−Day 28) and in the normal (Norm) digestive tube of the sea cucumber H. glaberrima. RT-PCR was performed as described in reference 15. Primer sequences for Gypsy-1_Hg and Gypsy-2_Hg were from reference 10. NADH dehydrogenasesubunit 5 was used as an internal control. The gel also includes a no-template control (“-” control) to test for contamination. Whereas Gypsy-1_Hg is not transcriptionally active in the normal gut but overexpressed in regeneration (in particular, at the early time points), Gypsy-2_Hg does not show any significant differences in expression level between the normal and regenerating digestive tube. This contrasts with expression of these genes in the radial organ complex regeneration in the same species (Fig. 3B in Mashanov et al.10), where both retroelements are barely detectable under the normal conditions, but show dramatic upregulation during regeneration.
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