ECB-ART-42692Dev Biol March 1, 2013; 375 (1): 92-104.
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Diversification of oral and aboral mesodermal regulatory states in pregastrular sea urchin embryos.
Specification of the non-skeletogenic mesoderm (NSM) in sea urchin embryos depends on Delta signaling. Signal reception leads to expression of regulatory genes that later contribute to the aboral NSM regulatory state. In oral NSM, this is replaced by a distinct oral regulatory state in consequence of Nodal signaling. Through regulome wide analysis we identify the homeobox gene not as an immediate Nodal target. not expression in NSM causes extinction of the aboral regulatory state in the oral NSM, and expression of a new suite of regulatory genes. All NSM specific regulatory genes are henceforth expressed exclusively, in oral or aboral domains, presaging the mesodermal cell types that will emerge. We have analyzed the regulatory linkages within the aboral NSM gene regulatory network. A linchpin of this network is gataE which as we show is a direct Gcm target and part of a feedback loop locking down the aboral regulatory state.
PubMed ID: 23261933
PMC ID: PMC3570723
Article link: Dev Biol
Genes referenced: gata6 gcml LOC100887844 LOC115919910 LOC115921237 nodall
Morpholinos: dll1 MO4 ehfl MO1 gata3 MO1 gata6 MO1 gata6 MO3 gata6 MO4 hmxl2 MO1 nodall MO3 prox1 MO1 prox1 MO2
References [+] :
Bolouri, Transcriptional regulatory cascades in development: initial rates, not steady state, determine network kinetics. 2003, Pubmed, Echinobase